Goat anti-Dog IgG (H&L), Unconjugated
AS16 3124 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG (H&L)
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Product Information
Immunogen
purified dog normal IgG isolated from pooled dog serum
Host
Goat
Clonality
Polyclonal
Purity
Protein G purified goat IgG in PBS pH 7.2.
Format
Lyophilized
Quantity
1 ml
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications
Immunoprecipitation (IP)
Recommended dilution
Immunoprecipitation, In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells, Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration
Reactivity
Confirmed reactivity
Dog IgG (heavy & light chains)
Not reactive in
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Application examples
Additional information
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
No preservative added.
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Background
Background
Goat anti-Dog IgG (H&L), is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Product citations
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