Goat anti-Dog IgG (H&L), Unconjugated
AS16 3124 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG (H&L)
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purified dog normal IgG isolated from pooled dog serum
Immunoprecipitation. In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration.
This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
No preservative added.
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Goat anti-Dog IgG (H&L), is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.