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Goat anti-Dog IgG (H&L), Unconjugated

AS16 3124 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG (H&L)

Goat anti-Dog IgG (H&L), Unconjugated  in the group Secondary Antibodies / Anti-Dog / Unconjugated at Agrisera AB (Antibodies for research) (AS16 3124)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

purified dog normal IgG isolated from pooled dog serum

Host Goat
Clonality Polyclonal
Purity Protein G purified IgG
Format Lyophilized in PBS, pH 7.2
Quantity 1 ml
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications Immunoprecipitation (IP)
Recommended dilution

Immunoprecipitation. In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration.

Reactivity

Confirmed reactivity Dog IgG (heavy & light chains)
Not reactive in

This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

No preservative added.

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.

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Background

Background

Goat anti-Dog IgG (H&L),  is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.

Product citations

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