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Goat anti-Dog IgG (H&L), FITC conjugated

AS16 3126 | Clonality: Polyclonal | Host: Goat | Reactivity: Dog IgG (H&L)

Goat anti-Dog IgG (H&L), FITC conjugated  in the group Secondary Antibodies / Anti-Dog / Fluorescent / FITC conjugated at Agrisera AB (Antibodies for research) (AS16 3126)



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Product Information

Immunogen

Purified normal IgG isolated from pooled dog serum

Host Goat
Clonality Polyclonal
Purity Protein G purified goat IgG in PBS pH 7.2.
Format Lyophilized
Quantity 2 ml
Reconstitution For reconstitution add 2 ml of sterile destilled water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (frozen tissue) (IHC), (in)directImmunofluorescence (IF)
Recommended dilution 1 : 20-1 : 120 (ICC) (IHC), (IF), The optimal working dilution should be established by titration by the end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal

Reactivity

Confirmed reactivity Dog IgG
Not reactive in This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion

Application examples

Additional information

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

No preservative added.

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.


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Background

Background

Goat anti-Dog IgG (H&L), FITC conjugated  is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.

Product citations

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