Goat anti-Human IgA (alpha chain), DyLightŪ 550 conjugated,min cross reactivity to bovine, mouse, rabbit serum
AS12 1899 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgA (α chain)
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Product Information
Immunogen
purified human IgA (alpha chain)
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified goat IgG.
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution
1 : 20-1 : 2000 for most applications
Reactivity
Application examples
Additional information
Additional information
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Based on immunoelectrophoresis, this antibody reacts with: heavy (α) chains on human IgA (α chain)
No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins serum proteins from bovine, mouse or rabbit.
Background
Background
Goat anti-human IgA (α chain) - DyLight®550 Conjugate, min cross reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to DyLight® 550, which binds to all human IgA (α chain) in immunological assays.
DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgA.DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product citations
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