Goat anti-Human IgA heavy (alpha chain), ALP conjugated

AS10 732 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgA heavy (alpha chain)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

purified human IgA, alpha chain

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA) , 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Human IgA heavy (alpha chains)

Predicted reactivity Human IgA Heavy (alpha chains)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information AP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to light chains or non-immunoglobulin human serum proteins based in immunoelectrophoresis

Background

Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA alpha chains in immunological assays.

Product citations

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background:	Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA alpha chains in immunological assays.
Clonality:	Polyclonal
Format:	Liquid
Host:	Goat
immunogen:	purified human IgA, alpha chain
Purity:	Immunogen affinity purified goat IgG.
Quantity:	1 mg
recommended dilution:	1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA) , 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Confirmed reactivity:	Human IgA heavy (alpha chains)
predicted reactivity:	Human IgA Heavy (alpha chains)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	AP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
additional information (application):	No reactivity is observed to light chains or non-immunoglobulin human serum proteins based in immunoelectrophoresis