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Goat anti-Human IgA+IgG+IgM, HRP conjugated

AS10 660 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgA+IgG+IgM

Goat anti-Human IgA+IgG+IgM, HRP conjugated  in the group Secondary Antibodies / Anti-Human / HRP (horse radish peroxidase) / IgA at Agrisera AB (Antibodies for research) (AS10 660)

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Product Information

Immunogen

affinity purified human IgA+IgG+IgM

Host Goat
Clonality Polyclonal
Purity Purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)

Reactivity

Confirmed reactivity Human IgA+IgG+IgM heavy chains and light chains on all Human immunoglobulins
Predicted reactivity Human IgA+IgG+IgM Heavy chains and Light chains on all Human immunoglobulins
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

No reactivity to non immunoglobulin human proteins based on immunoelectrophoresis

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Background

Background

Goat anti-human IgA+IgG+IgM  is a secondary antibody conjugated to HRP which binds to human immunoglobulins in immunological assays.

Product citations

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