Goat anti-Human IgA+IgG+IgM, HRP conjugated

AS10 660 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgA+IgG+IgM


product information
Background		Goat anti-human IgA+IgG+IgM is a secondary antibody conjugated to HRP which binds to human immunoglobulins in immunological assays.
Immunogen		affinity purified human IgA+IgG+IgM
Host		Goat
Clonality		Polyclonal
Clone
Purity		Purified goat IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related products
Additional information		HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.

application information
Recommended dilution		1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Expected \| apparent MW
Confirmed reactivity		Human IgA+IgG+IgM heavy chains and light chains on all Human immunoglobulins
Predicted reactivity		Human IgA+IgG+IgM Heavy chains and Light chains on all Human immunoglobulins
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		No reactivity to non immunoglobulin human proteins based on immunoelectrophoresis
Selected references		to be added when available

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com