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Goat anti-Human IgG Fc, FITC conjugated

AS10 977  | Clonality: Polyclonal  |  Host: Goat |  Reactivity: Human IgG Fc
Goat anti-Human IgG Fc, FITC conjugated  in the group Secondary Antibodies / Anti-Human / Fluorescent / IgG at Agrisera AB (Antibodies for research) (AS10 977)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Liquid
Quantity 2 mg
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 50-1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.

Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins. Minimum cross-reactivity is observed to human IgG, F(ab)'2 fragment based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

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Background

Background Goat anti-human IgG Fc is a  FITC conjugated secondary antibody which binds to human IgG upsilon in immunological assays. FITC (fluorescein-5-isothiocyanate) has  Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgG.

Product citations

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