Goat anti-Human IgG Fc, HRP conjugated, min, reactivity to human IgA and IgM

Product no: AS10 664

AS10 664 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG Fc

10 % until end of 2023. Use discount code: Conj10

206
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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    affinity purified human IgG Fc part

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 100 000 (WB)
  • Reactivity
  • Confirmed reactivity: Human IgG heavy chain Fc based on immunoelectrophoresis
    Predicted reactivity: Human IgG Fc
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

    0.1 % (v/v) of Kathon CG is used as preservative.

    Additional information (application):

    No reactivity to serum proteins from bovine or rabbit, No reactivity is observed to human IgA or IgM based on immunoelectrophoresis

  • Background
  • Background:

    Goat anti-human IgG Fc  is a secondary antibody conjugated to HRP which binds to human Fc part of IgG in immunological assays. The antibody is absorbed agains human IgA and IgM

  • Protocols
  • Antibody protocols
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