Goat anti-Human IgG Fc, HRP conjugated, min. reactivity to human IgA and IgM

AS10 664 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG Fc


product information
Background		Goat anti-human IgG Fc is a secondary antibody conjugated to HRP which binds to human Fc part of IgG in immunological assays. The antibody is absorbed agains human IgA and IgM
Immunogen		affinity purified human IgG Fc part
Host		Goat
Clonality		Polyclonal
Clone
Purity		Affinity purified IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related products		antibodies to human IgG
Additional information		HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.

application information
Recommended dilution		1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 100 000 (WB)
Expected \| apparent MW
Confirmed reactivity		Human IgG heavy chain Fc based on immunoelectrophoresis
Predicted reactivity		Human IgG Fc
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		No reactivity to serum proteins from bovine or rabbit, No reactivity is observed to human IgA or IgM based on immunoelectrophoresis
Selected references		to be added when available

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