Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated

AS10 779 | Clonality: Polyclonal | Host: Goat | Reactivity:Human IgG (H&L), F(ab)'2 fragment

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

purified human IgG (H&L)

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 0,55 ml of sterile water

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity Human IgG (H&L), F(ab)'2 fragment

Predicted reactivity Human IgG (H&L), F(ab)'2 fragment

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

Background

Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.

Product citations

additional information:	HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
additional information (application):	This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Confirmed reactivity:	Human IgG (H&L), F(ab)'2 fragment
predicted reactivity:	Human IgG (H&L), F(ab)'2 fragment
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	purified human IgG (H&L)
Purity:	Immunogen affinity purified goat IgG.
Quantity:	0,5 mg
recommended dilution:	The optimal working dilution should be determined by the investigator
Reconstitution:	For reconstitution add 0,55 ml of sterile water
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
background:	Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.

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