Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum

Name: Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum
SKU: AS10 781
Price: 206 EUR
Availability: InStock

AS10 781 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (H&L), F(ab)'2 fragment

10 % until end of 2023. Use discount code: Conj10

Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum in the group Secondary Antibodies / Anti-Human / ALP (alkaline phosphatase) / IgG at Agrisera AB (Antibodies for research) (AS10 781)

Product Information

Immunogen

Purified human IgG (H&L)

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Liquid

Quantity 0,5 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Human IgG (H&L), F(ab)'2 fragment

Predicted reactivity Human IgG (H&L), F(ab)'2 fragment

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

Purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.

This antibody reacts with the heavy (gamma) chains on human IgG and light chains on all human immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins and serum from mouse, rabbit of bovine serum proteins based on immunoelectrophoresis.

Background

Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.

Product citations

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immunogen:	Purified human IgG (H&L)
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Liquid
Quantity:	0,5 mg
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Confirmed reactivity:	Human IgG (H&L), F(ab)'2 fragment
predicted reactivity:	Human IgG (H&L), F(ab)'2 fragment
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative. Purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
additional information (application):	This antibody reacts with the heavy (gamma) chains on human IgG and light chains on all human immunoglobulins based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins and serum from mouse, rabbit of bovine serum proteins based on immunoelectrophoresis.
background:	Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.