Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum
AS10 781 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (H&L), F(ab)'2 fragment
- Product Info
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Immunogen: Purified human IgG (H&L)
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Liquid Quantity: 0.5 mg Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) - Reactivity
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Confirmed reactivity: Human IgG (H&L), F(ab)'2 fragment Predicted reactivity: Human IgG (H&L), F(ab)'2 fragment Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA, Protease/IgG free. 0.05% (w/v) of sodium azide is added as preservative.
Purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
Additional information (application): This antibody reacts with the heavy (gamma) chains on human IgG and light chains on all human immunoglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin human serum proteins and serum from mouse, rabbit of bovine serum proteins based on immunoelectrophoresis.
- Background
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Background: Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
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