showForm Goat anti-human IgG + IgA + IgM, F(ab)'2 fragment,HRP conjugate

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Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated

AS10 1039 | Clonality: Polyclonal  |  Host: Goat |  Reactivity: Human IgG + IgA + IgM

Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated in the group Secondary Antibodies / Anti-Human / HRP (horse radish peroxidase) / IgA at Agrisera AB (Antibodies for research) (AS10 1039)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG, F(ab)'2 fragment
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution add 0.55 ml of sterile water
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on human IgG + IgA + IgM, and the light chains on all human immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.

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Background

Background Goat anti-human IgG + IgA + IgM, HRP conjugated is a F(ab)'2 fragment, of a secondary antibody which binds to human IgG+IgA+IgM in immunological assays.  Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

Product citations

Selected references Wielkoszynski et al. (2018). Novel diagnostic ELISA test for discrimination between infections with Yersinia enterocolitica and Yersinia pseudotuberculosis. Eur J Clin Microbiol Infect Dis. 2018 Dec;37(12):2301-2306. doi: 10.1007/s10096-018-3373-9.

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