Goat anti-Human IgA + IgG + IgM, F(ab)'2 fragment, ALP conjugated

AS10 1222 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgA + IgG + IgM F(ab)'2 fragment

Product Information

Host Goat

Clonality Polyclonal

Purity Affinity purified IgG

Format Liquid

Quantity 0.5 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy and light on human IgA + IgG + IgM based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

Related products

Related products other ALP conjugated secondary antibodies to human immunoglobulins

Background

Background Goat anti-human IgA + IgG + IgM, ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to human IgA and IgG and IgM F(ab)'2 fragment in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

Product citations

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