Goat anti-Human IgM (µ chain), F(ab)'2 fragment, HRP conjugated

Product no: AS10 1009

AS10 1009  | Clonality: Polyclonal  |  Host: Goat  |  Reactivity: Human IgM, µ chain

10 % until end of 2023. Use discount code: Conj10

246
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Delivery:  3-6 business days
  • Product Info
  • Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG, F(ab)'2 fragment.
    Format: Lyophilized
    Quantity: 0,5 mg
    Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Recommended dilution: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis.

    Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

  • Background
  • Background: Goat anti-human IgM, µ chain, is a F(ab)'2 fragment, of HRP conjugated secondary antibody which binds to human IgM, µ chain in immunological assays. Antibodies are affinity purified using solid phase human IgM.
  • Protocols
  • Antibody protocols
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