Goat anti-Mouse IgG (H&L), DyLight® 488 conjugated, min, cross-reactivity to human IgG or serum proteins

Name: Goat anti-Mouse IgG (H&L), DyLight® 488 conjugated, min, cross-reactivity to human IgG or serum proteins
SKU: AS09 637
Price: 175 EUR
Availability: InStock

Product no: AS09 637

AS09 637 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)

175 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	Purified Mouse IgG, whole molecule
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution:	1 : 20-1 : 2000 for most applications

Additional Information

Additional information:

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulins

No reactivity is observed to: non-immunoglobulin mouse serum proteins, human IgG or serum proteins

Background

Background:

Goat anti-mouse IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to human IgG or serum proteins is a secondary antibody conjugated to DyLight® 488, which binds to Mouse IgG (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase mouse IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product Citations

Selected references:

Wang et al. (2016). Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories. Dev Biol. 2016 Aug 1;416(1):225-34. doi: 10.1016/j.ydbio.2016.05.020. Epub 2016 May 19
Liu et al. (2016). Fold formation at the compartment boundary of Drosophila wing requires Yki signaling to suppress JNK dependent apoptosis. Sci Rep. 2016 Nov 29;6:38003. doi: 10.1038/srep38003.

Protocols
Antibody protocols
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