Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugated

Name: Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugated
SKU: AS10 1139
Price: 233 EUR
Availability: InStock

AS10 1139 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugated in the group Secondary Antibodies / Anti-Mouse / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1139)

Product Information

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified IgG, F(ab)'2 fragment.

Format Liquid

Quantity 0,5 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

Background

Background Goat anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to mouse IgG in immunological assays. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

Product citations

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Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified IgG, F(ab)'2 fragment.
Format:	Liquid
Quantity:	0,5 mg
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
recommended dilution:	1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB)
additional information:	This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
background:	Goat anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to mouse IgG in immunological assays. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.