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Goat anti-Mouse IgG (H&L), FITC conjugated, min. cross-reactivity to human IgG/serum

AS10 962 | Clonality: Polyclonal  | Host: Goat | Reactivity: Mouse IgG (H&L)

Goat anti-Mouse IgG (H&L), FITC conjugated, min. cross-reactivity to human IgG/serum in the group Secondary Antibodies / Anti-Mouse / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 962)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Liquid
Quantity 2 mg
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 50 1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

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Background

Background Goat anti-mouse IgG (H&L) is a FITC conjugated secondary antibody which is reacting with all mouse IgG (H&L) in immunological assays. Min. cross-reactivity to human IgG/serum. FITC (fluorescein-5-isothiocyanate) has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase mouse IgG.

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