Goat anti-Mouse IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, goat,human, rabbit, rat IgG
AS10 964 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG (H&L)
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Product Information
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified IgG
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage
Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1 : 20-1 : 2000 depending upon the application (immunolocalization or western blot).
Reactivity
Application examples
Additional information
Additional information
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins as well as bovine, goat, human, rabbit or rat IgG.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Background
Background
Goat anti-mouse IgG (H&L) is a secondary antibody HRP conjugated which binds to all mouse IgGs in immunological assays. It is specially adsorbed against bovine,goat,human,rabbit,rat IgG.
Product citations
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