Goat anti-Rabbit IgG Fc, HRP conjugated

Name: Goat anti-Rabbit IgG Fc, HRP conjugated
SKU: AS10 858
Price: 165 EUR
Availability: InStock

AS10 858 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG Fc (two heavy chains with constant domains)

10 % until end of 2023. Use discount code: Conj10

Goat anti-Rabbit IgG Fc, HRP conjugated in the group Secondary Antibodies / Anti-Rabbit / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 858)

Product Information

Immunogen

Purified Rabbit IgG

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity rabbit IgG Fc (two heavy chains with constant domains)

Predicted reactivity Rabbit IgG Fc (two Heavy chains with constant domains)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase

This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.

No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.

Background

Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.

Product citations

immunogen:	Purified Rabbit IgG
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Tested applications:	ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	The optimal working dilution should be determined by the investigator
Confirmed reactivity:	rabbit IgG Fc (two heavy chains with constant domains)
predicted reactivity:	Rabbit IgG Fc (two Heavy chains with constant domains)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
additional information (application):	This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis. No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis. This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.
background:	Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.

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