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Goat anti-Rabbit IgG (H&L), ALP conjugated, min. cross-reactivity to bovine,goat,human, mouse,rat IgG

AS10 1086  | Clonality: Polyclonal  |  Host: Goat  |  Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L), ALP conjugated, min. cross-reactivity to bovine,goat,human, mouse,rat IgG  in the group Secondary Antibodies / Anti-Rabbit / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS10 1086)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 500 -1 : 2000 (ELISA), 1 : 50 -1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500 -1 : 2000 (WB)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with: heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins  IgG from bovine, goat, human, mouse or rat. 

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

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Background

Background Goat anti-rabbit IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which binds all rabbit IgGs in immunological assays. Antibody is adsorbed againstbovine,goat,human,mouse,rat IgG and affinity purified using solid phase rabbit IgG.

Product citations

Selected references Wężowicz et al. (2017). Interactions of arbuscular mycorrhizal and endophytic fungi improve seedling survival and growth in post-mining waste. Mycorrhiza. 2017 Mar 20. doi: 10.1007/s00572-017-0768-x.