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Goat anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse, rat serum

AS10 1468 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
Goat anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse, rat serum in the group Secondary Antibodies / Anti-Rabbit / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1468)

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Product Information

Immunogen Purified rabbit IgG (H&L)
Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.

Reactivity

Application examples

Additional information

Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat

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Background

Background Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).

Product citations

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