Purified normal IgA isolated from pooled rat serum,
Purified by salt precipitation, followed by a ion-exchange chromatography with DEAE Sepharose, in PBS pH 7.2.
For reconstitution add 1 ml of sterile destilled water, Spin down to remove insoluble particles
Store at +4°C, for long time storage, store at -20°C.
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
The optimum working dilution should be established by titration before being used