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Goat anti-Guinea pig IgG (H&L), HRP conjugated

AS10 653 | Clonality: Polyclonal | Host: Goat | Reactivity: Guinea pig IgG (H&L)

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Goat anti-Guinea pig IgG (H&L), HRP conjugated in the group Secondary Antibodies / Anti-Guinea Pig / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 653)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Purified Guinea pig IgG, whole molecule

Host Goat
Clonality Polyclonal
Purity Affinity purified using solid phase guinea pig IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)

Reactivity

Confirmed reactivity Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins
Not reactive in Non-immunoglobulin guinea pig serum proteins

Application examples

Additional information

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free with 0.1 % (v/v) of Kathon CG as preservative.

Related products

Related products

AS09 619 | Goat anti-rat IgG (H&L) biotin conjugated
AS06 620 | Goat anti-rat IgG (H&L) ALP(alkaline phosphatase) conjugated

Collection of secondary antibodies

Background

Background

Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.

Product citations

Selected references Jin et al. (2019).  YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914.

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