Goat anti-Guinea pig IgG (H&L), HRP conjugated

AS10 653 | Clonality: Polyclonal | Host: Goat | Reactivity: Guinea pig IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

Purified Guinea pig IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)

Reactivity

Confirmed reactivity Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins

Not reactive in Non-immunoglobulin guinea pig serum proteins

Application examples

Additional information

Additional information HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of Kathon CG as preservative

Background

Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.

Product citations

Selected references Jin et al. (2019). YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914.

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Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	Purified Guinea pig IgG, whole molecule
Purity:	Immunogen affinity purified goat IgG.
Quantity:	1 mg
recommended dilution:	1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Confirmed reactivity:	Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins
not reactive in:	Non-immunoglobulin guinea pig serum proteins
All references:	Jin et al. (2019). YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914.
additional information:	HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of Kathon CG as preservative
background:	Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.