Goat anti-Guinea pig IgG (H&L), HRP conjugated
AS10 653 | Clonality: Polyclonal | Host: Goat | Reactivity: Guinea pig IgG (H&L)
10 % until end of 2023. Use discount code: Conj10

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
Purified Guinea pig IgG, whole molecule
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified goat IgG.
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Storage
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Reactivity
Confirmed reactivity
Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins
Not reactive in
Non-immunoglobulin guinea pig serum proteins
Application examples
Additional information
Additional information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of Kathon CG as preservative
Background
Background
Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.
Product citations
Selected references
Jin et al. (2019). YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914.
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