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Anti-GST-tag clone 21, mouse monoclonal
Product no: AS20 4514
AS20 4514 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tag
253
€
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Delivery:
3-6 business days
- Product Info
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Sub class: IgG Immunogen: Recombinant GST of Schistosoma japonicum UniProt: P08515 Host: Mouse Clonality: Monoclonal Purity: Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized. Format: Liquid at 1 mg/ml. Quantity: 100 µg Storage: Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1: 500 (IF), 1: 200 (IP), 1: 1000 - 1: 3000 (WB) - Reactivity
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Confirmed reactivity: GST-tagged recombinant proteins - Application Examples
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HeLa cell extracts, 10 μg/well
1. Control cells 45kDa
2. Cells transformed with COXFA4l2-GST expressing plasmid
3. Cells transformed with COXFA4-GST expressing plasmid
4. Cells transformed 20kDa with COXFA4l1-GST expressing plasmid
Transfer: nitrocellulose.
The anti-GST antibody was used at 1:1000 dilution 1h/RT incubation in TBS-T, following the incubation with a secondary antibody goat anti-mouse HRP conjugated at 1: 20 000 1h/RT incubation and reaction was visualized using chemiluminescence, following manufactur's recommendations. - Background
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Background: GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. - Product Citations
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Selected references: - Protocols
- Antibody protocols
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