Anti-GST-tag clone 21, mouse monoclonal

Product no: AS20 4514

AS20 4514 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tag

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Delivery:  3-6 business days
  • Product Info
  • Sub class: IgG
    Immunogen: Recombinant GST of Schistosoma japonicum UniProt: P08515
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.
    Format: Liquid at 1 mg/ml.
    Quantity: 100 g
    Storage: Store at -20C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 1: 500 (IF), 1: 200 (IP), 1: 1000 - 1: 3000 (WB)
  • Reactivity
  • Confirmed reactivity: GST-tagged recombinant proteins
  • Application Examples
  • Western blot using anti-GST monoclonal antibodies
    HeLa cell extracts, 10 μg/well
    1. Control cells 45kDa
    2. Cells transformed with COXFA4l2-GST expressing plasmid
    3. Cells transformed with COXFA4-GST expressing plasmid
    4. Cells transformed 20kDa with COXFA4l1-GST expressing plasmid
    Transfer: nitrocellulose. 
    The anti-GST antibody was used at 1:1000 dilution 1h/RT incubation in TBS-T, following the incubation with a secondary antibody goat anti-mouse HRP conjugated at 1: 20 000 1h/RT incubation and reaction was visualized using chemiluminescence, following manufactur's recommendations.
  • Background
  • Background: GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • Product Citations
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  • Protocols
  • Antibody protocols
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