GST-tag clone 21, mouse monoclonal

Name: GST-tag clone 21, mouse monoclonal
Brand: IKP
SKU: AS20 4514
Price: 233 EUR
Availability: InStock

AS20 4514 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tag

GST-tag clone 21, mouse monoclonal in the group Alla produktegenskaper at Agrisera AB (Antibodies for research) (AS20 4514)

Product Information

Immunogen Recombinant GST of Schistosoma japonicum UniProt: P08515

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG

Purity Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.

Format Liquid at 1 mg/ml.

Quantity 100 µg

Storage Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1: 500 (IF), 1: 200 (IP), 1: 1000 - 1: 3000 (WB)

Reactivity

Confirmed reactivity GST-tagged recombinant proteins

Application examples

Application examples Western blot using anti-GST monoclonal antibodies

HeLa cell extracts, 10 μg/well
1. Control cells 45kDa
2. Cells transformed with COXFA4l2-GST expressing plasmid
3. Cells transformed with COXFA4-GST expressing plasmid
4. Cells transformed 20kDa with COXFA4l1-GST expressing plasmid
Transfer: nitrocellulose.
The anti-GST antibody was used at 1:1000 dilution 1h/RT incubation in TBS-T, following the incubation with a secondary antibody goat anti-mouse HRP conjugated at 1: 20 000 1h/RT incubation and reaction was visualized using chemiluminescence, following manufactur's recommendations.

Additional information

Background

Background GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Product citations

Selected references

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immunogen:	Recombinant GST of Schistosoma japonicum UniProt: P08515
Sub class:	IgG
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.
Format:	Liquid at 1 mg/ml.
Quantity:	100 µg
storage:	Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
recommended dilution:	1: 500 (IF), 1: 200 (IP), 1: 1000 - 1: 3000 (WB)
Confirmed reactivity:	GST-tagged recombinant proteins
Picture (footer):	HeLa cell extracts, 10 μg/well 1. Control cells 45kDa 2. Cells transformed with COXFA4l2-GST expressing plasmid 3. Cells transformed with COXFA4-GST expressing plasmid 4. Cells transformed 20kDa with COXFA4l1-GST expressing plasmid Transfer: nitrocellulose. The anti-GST antibody was used at 1:1000 dilution 1h/RT incubation in TBS-T, following the incubation with a secondary antibody goat anti-mouse HRP conjugated at 1: 20 000 1h/RT incubation and reaction was visualized using chemiluminescence, following manufactur's recommendations.
background:	GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
All references: