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GST-tag clone 21, mouse monoclonal

AS20 4514 | Clonality: Monoclonal | Host: Mouse | Reactivity: GST-tag

GST-tag clone 21, mouse monoclonal  in the group Antibodies for Human/Animal  / Tag Antibodies / GST at Agrisera AB (Antibodies for research) (AS20 4514)

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Product Information

Immunogen Recombinant GST of Schistosoma japonicum UniProt: P08515
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG
Purity Purified by Protein A affinity
Format Liquid in PBS, 50 % glycerol, filter sterilized, azide and protein carrier free, at concentration of 1 mg/ml
Quantity 100 µg
Storage Store at -20°C. Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 500 (IF), 1: 200 (IP), 1: 1000 - 1: 3000 (WB)

Reactivity

Confirmed reactivity GST-tagged recombinant proteins

Application examples

Application examples Western blot using anti-GST monoclonal antibodies
HeLa cell extracts, 10 μg/well
1. Control cells 45kDa
2. Cells transformed with COXFA4l2-GST expressing plasmid
3. Cells transformed with COXFA4-GST expressing plasmid
4. Cells transformed 20kDa with COXFA4l1-GST expressing plasmid
Transfer: nitrocellulose. 
The anti-GST antibody was used at 1:1000 dilution 1h/RT incubation in TBS-T, following the incubation with a secondary antibody goat anti-mouse HRP conjugated at 1: 20 000 1h/RT incubation and reaction was visualized using chemiluminescence, following manufactur's recommendations.

Additional information

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Background

Background GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

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