H3 | Histone H3, Biotin-conjugated (40 µg)

Product no: AS10 710B

AS10 710B | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays, Ch. reinhardtii | cellular [compartment marker] of nucleoplasm

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
    Format: Liquid
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 - 1: 5000 (WB)
    Expected | apparent MW:

    15 | 17 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity:

    Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Nannochloropsis gaditana, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.


    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii
  • Background
  • Background:

    Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection



    Preparation of cytosolic and nuclear protein fractions


    1. Prepare protoplasts from 50 ml Arabidopsis thaliana cell culture according to the protocol of PEG transfection.
    2. Resuspend protoplasts in 10 ml GH buffer and keep the solution on ice for 10 min.
    GH buffer: 100mM glycine
    0.1% Hexylene glycol
    0.37M (4.7% w/v) saccharose
    0.3mM Spermine
    1.0mM Spermidine
    pH 8.3 with Ca(OH)2
    3. To release nuclei add Triton X100 to a final concentration of 0.1%. Pipetting gently up and down several
    times with a plastic pipette might be necessary to lyse cells.
    4. After 5min sediment nuclei by centrifugation at 1000 xg for 15 min at 4°C. Save supernatant as the
    cytoplasmic fraction. Wash the pelleted nuclei two times with GHT (GH+0.1% TX100) then finally
    resuspended in a suitable volume of extraction buffer + protease inhibitors.

    Courtesy Dr. Laszlo Bako, Umeå Plant Science Centre, Sweden

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Accessories

AS10 710-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays, Ch. reinhardtii | cellular [compartment marker] of nucleoplasm

464 €
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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 16 €

AS10 710 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, H. vulgare, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays | cellular [compartment marker] of nucleoplasm

302 €
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Buy 2 items of this product for 226.00 €/items
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