H3K9ac | Histone H3, acetylated lysine 9

AS16 3198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Human, Mouse, P. falciparum, Populus sp.

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS with 0.05 % sodium azide

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Chromatin immunoprecipitation (ChIP/ChIPseq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 2-5 µg/IP (ChIP), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)

Expected | apparent MW 15.4 | 17 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, human

Predicted reactivity Mouse, Plasmodium falciparum, Populus sp.
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Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-H3K9ac | Histone H3, acetylated lysine 9 polyclonal antibodies

Dot Blot analysis was performed to test the cross reactivity of anti-H3K9ac antibodies with peptides containing other histone modifications and the unmodified H3K9 sequence. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest. The antibody recognizes the H3K9 acetylation, both in the presence and the absence of the K14 acetylation

Additional information

Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.

Related products

Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

Product citations

Selected references Bellegarde et al. (2018). Polycomb Repressive Complex 2 attenuates the very high expression of the Arabidopsis gene NRT2.1. Sci Rep. 2018 May 21;8(1):7905. doi: 10.1038/s41598-018-26349-w.