Name:
Phone:
E-mail:
Message


 


H3K9ac | Histone H3, acetylated lysine 9

AS16 3198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thalianaHuman, Mouse, P. falciparum, Populus sp. 
H3K9ac | Histone H3, acetylated lysine 9 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3198)

DATA SHEET IN PDF

Qty: 
391
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS with 0.05 % sodium azide
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP/ChIPseq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 2-5 µg/IP (ChIP), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
Expected | apparent MW 15.4 |  17 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, human
Predicted reactivity Mouse, Plasmodium falciparum, Populus sp.
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-H3K9ac | Histone H3, acetylated lysine 9  polyclonal antibodies
Dot Blot analysis was performed to test the cross reactivity of anti-H3K9ac antibodies with peptides containing other histone modifications and the unmodified H3K9 sequence. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest. The antibody recognizes the H3K9 acetylation, both in the presence and the absence of the K14 acetylation

Additional information

Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.

Related products

Related products AS16 3195 | Anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade), rabbit antibodies

AS16 3190-10 | Anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) (10 µg), rabbit antibodies

AS16 3187 | Anti-H3K56me1 | Histone H3 (monomethyl Lys56), polyclonal antibodies
AS16 3182
| Anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies
AS16 3183 | Anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies

AS16 3181 | Anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies
AS16 3168 | Anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies
AS16 3169 | Anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies
AS16 3172 | Anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodies

AS15 2855 | Anti-H3 | histone H3, chicken polyclonal antibodies
AS10 710 | Anti-H3 | histone H3, rabbit polyclonal antibodies
AS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710

collection of antibodies to epigenetics

Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.

Product citations

Selected references Bellegarde et al. (2018). Polycomb Repressive Complex 2 attenuates the very high expression of the Arabidopsis gene NRT2.1. Sci Rep. 2018 May 21;8(1):7905. doi: 10.1038/s41598-018-26349-w.

Related products: H3K9ac | Histone H3, acetylated lysine 9

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection...
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
...
190 €
AS16 3197 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Solanum lycopersicum, Populus...
391 €
AS10 710 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, S. lyco...
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items