Anti-H3K9ac | Histone H3, acetylated lysine 9

Product no: AS16 3198
AS16 3198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thalianaHuman, Mouse, P. falciparum, Populus sp. 
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS. Contains 0.05% sodium azide.
    Format: Liquid
    Quantity: 50 µg
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: 2-5 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
    Expected | apparent MW: 15.4 |  17 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, human
    Predicted reactivity: Chlamydomonas reinhardii, mouse, Plasmodium falciparum, Populus sp.
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using anti-H3K9ac | Histone H3, acetylated lysine 9 polyclonal antibodies
    Dot Blot analysis was performed to test the cross reactivity of anti-H3K9ac antibodies with peptides containing other histone modifications and the unmodified H3K9 sequence. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest. The antibody recognizes the H3K9 acetylation, both in the presence and the absence of the K14 acetylation
  • Additional Information
  • Additional information: Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
  • Background
  • Background: Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
  • Product Citations
  • Selected references: Bellegarde et al. (2018). Polycomb Repressive Complex 2 attenuates the very high expression of the Arabidopsis gene NRT2.1. Sci Rep. 2018 May 21;8(1):7905. doi: 10.1038/s41598-018-26349-w.
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