H3K9ac | Histone H3, acetylated lysine 9
AS16 3198 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Human, Mouse, P. falciparum, Populus sp.
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS with 0.05 % sodium azide
Format
Liquid
Quantity
50 µg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Chromatin immunoprecipitation (ChIP/ChIPseq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
2-5 µg/IP (ChIP), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
Expected | apparent MW
15.4 | 17 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, human
Predicted reactivity
Mouse, Plasmodium falciparum, Populus sp.
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
application example
Dot Blot analysis was performed to test the cross reactivity of anti-H3K9ac antibodies with peptides containing other histone modifications and the unmodified H3K9 sequence. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest. The antibody recognizes the H3K9 acetylation, both in the presence and the absence of the K14 acetylation
Dot Blot analysis was performed to test the cross reactivity of anti-H3K9ac antibodies with peptides containing other histone modifications and the unmodified H3K9 sequence. 100 to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest. The antibody recognizes the H3K9 acetylation, both in the presence and the absence of the K14 acetylation
Additional information
Additional information
Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
Background
Background
Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Product citations
Selected references
Bellegarde et al. (2018). Polycomb Repressive Complex 2 attenuates the very high expression of the Arabidopsis gene NRT2.1. Sci Rep. 2018 May 21;8(1):7905. doi: 10.1038/s41598-018-26349-w.
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