H3 | Histone 3 core | (monoclonal)
AS16 3197 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Solanum lycopersicum, Populus sp., Zea mays
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein.
Host
Mouse
Clonality
Monoclonal
Purity
Protein A purified in PBS with 0.05 % sodium azide.
Format
Liquid
Quantity
50 µg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Chromatin immunoprecipitation (ChIP), Western blot (WB)
Recommended dilution
1-2 µg/IP (ChIP), 1 : 1000 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Human
Predicted reactivity
Solanum lycopersicum, Populus sp., Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
application example
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). The anti-H3 core antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). The anti-H3 core antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
Additional information
Additional information
Antibody has been purified using Protein A.
Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
Background
Background
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
Product citations
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