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H3 | Histone 3 core | (monoclonal)

AS16 3197 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Solanum lycopersicum, Populus sp., Zea mays
H3 | Histone 3 core | (monoclonal) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3197)
H3 | Histone 3 core | (monoclonal)



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Product Information

Immunogen KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein.
Host Mouse
Clonality Monoclonal
Purity Protein A purified in PBS with 0.05 % sodium azide.
Format Liquid
Quantity 50 ĩg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Chromatin immunoprecipitation (ChIP), Western blot (WB)
Recommended dilution 1-2 µg/IP (ChIP), 1 : 1000 (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Solanum lycopersicum, Populus sp., Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-H3core | (monoclonal) antibodies

Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). The anti-H3 core antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.

Additional information

Additional information Antibody has been purified using Protein A.

Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.

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Background

Background

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

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