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H3 | Histone 3 core | (monoclonal)

AS16 3197 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Solanum lycopersicum, Populus sp., Zea mays
H3 | Histone 3 core | (monoclonal) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3197)

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Product Information

Immunogen KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein.
Host Mouse
Clonality Monoclonal
Purity Protein A purified in PBS with 0.05 % sodium azide.
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Western blot (WB)
Recommended dilution 1-2 µg/IP (ChIP), 1 : 1000 (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Solanum lycopersicum, Populus sp., Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-H3core | (monoclonal) antibodies

Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). The anti-H3 core antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.

Additional information

Additional information Antibody has been purified using Protein A.

Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.

Related products

Related products AS16 3195 | Anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade), rabbit antibodies

AS16 3190-10 | Anti-H3K4me3 | histone H3, trimethylated lysine 4 (H3K4me3) (10 µg), rabbit antibodies

AS16 3187 | Anti-H3K56me1 | Histone H3 (monomethyl Lys56), polyclonal antibodies
AS16 3182
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AS16 3183 | Anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies

AS16 3181 | Anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies
AS16 3168 | Anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies
AS16 3169 | Anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies
AS16 3172 | Anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodies

AS15 2855 | Anti-H3 | histone H3, chicken polyclonal antibodies
AS10 710 | Anti-H3 | histone H3, rabbit polyclonal antibodies
AS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710

collection of antibodies to epigenetics

Background

Background

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

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