IKP

H3 | Histone 3 core | (monoclonal)

Product no: AS16 3197
AS16 3197 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Solanum lycopersicum, Populus sp., Zea mays
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein.
    Host: Mouse
    Clonality: Monoclonal
    Purity: Protein A purified in PBS with 0.05 % sodium azide.
    Format: Liquid
    Quantity: 50 g
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Chromatin immunoprecipitation (ChIP), Western blot (WB)
    Recommended dilution: 1-2 µg/IP (ChIP), 1 : 1000 (WB)
    Expected | apparent MW:

    15 kDa

  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Solanum lycopersicum, Populus sp., Zea mays
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using anti-H3core | (monoclonal) antibodies

    Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively). The anti-H3 core antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk.
  • Additional Information
  • Additional information: Antibody has been purified using Protein A.

    Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
  • Background
  • Background:

    Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

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