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H3 | Histone H3 (chicken antibody)

AS15 2855 | Clonality: Polyclonal | Host: Chicken | Reactivity: A. thaliana, C. reinhardtii, L. esculentum | cellular [compartment marker] of nucleoplasm

H3 | Histone H3 (chicken antibody) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Nuclear signaling at Agrisera AB (Antibodies for research) (AS15 2855)

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host Chicken
Clonality Polyclonal
Purity Total IgY
Format Liquid in PBS, pH 8.5 with 0.02 % sodium azide
Quantity 50 µl
Storage Store at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2500 (WB)
Expected | apparent MW

15 | 17 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Lycopersicum esculentum
Predicted reactivity

Arabidopsis thaliana, Brassica napus, Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Hordeum vulgare, Medicago sativa, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pinus pinaster, Pisum sativum, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Triticum aestivum, Zea mays, Vicia faba, Vitis vinifera, Volvox sp.


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-plant histone 3, chicken antibody
From left to right: 2 wells of nuclear + cytoplasmic enriched protein, 2 wells of total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (AS11 1827, Goat anti-Chicken IgY (H&L), DyLight® 800 conjugated, Agrisera) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom

Additional information

Additional information Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

Related products

Related products AS11 1804 | Anti-RNA polymerase II subunit B1, rabbit antibodies
AS16 3197 | Anti-H3 | Histone 3 core, monoclonal antibodies
AS10 710-ALP | Anti-H3 | Histone H3, ALP-conjugated (40 µg)
AS10 710-HRP | Anti-H3 | Histone H3, HRP-conjugated (40 µg) 

collection of antibodies to DNA/RNA/cell cycle
Plant and algal protein extraction buffer

Background

Background

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Product citations

Selected references Chung et al. (2019) Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression. Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x.

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