H3 | Histone H3 (chicken antibody)

Name: H3 | Histone H3 (chicken antibody)
SKU: AS15 2855
Price: 302 EUR
Availability: InStock

AS15 2855 | Clonality: Polyclonal | Host: Chicken | Reactivity: A. thaliana, C. reinhardtii, L. esculentum, T. aestivum | cellular [compartment marker] of nucleoplasm

H3 | Histone H3 (chicken antibody) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Nuclear signaling at Agrisera AB (Antibodies for research) (AS15 2855)

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host Chicken

Clonality Polyclonal

Purity Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format Liquid

Quantity 50 µl

Storage Store at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2500 (WB)

Expected | apparent MW

15 | 17 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Lycopersicum esculentum, Triticum aestivum

Predicted reactivity

Arabidopsis thaliana, Brassica napus, Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Hordeum vulgare, Medicago sativa, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pinus pinaster, Pisum sativum, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Triticum aestivum, Zea mays, Vicia faba, Vitis vinifera, Volvox sp.

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-plant histone 3, chicken antibody

From left to right: 2 wells of nuclear + cytoplasmic enriched protein, 2 wells of total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (AS11 1827, Goat anti-Chicken IgY (H&L), DyLight® 800 conjugated, Agrisera) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom

Reactant: Chlamydomonas reinhardtii (Green Alga)

Application: Western Blotting

Pudmed ID: 31366981

Journal: Sci Rep

Figure Number: 2C

Published Date: 2019-07-31

First Author: Chung, B. Y. W., Valli, A., et al.

Impact Factor: 4.13

Open Publication

Expression and differential localisation of AGO2 and AGO3. (a) Antigen sites utilised for peptide-based antibody production. (b) Western blots for total and ?-2/3-PAZ-immunoprecipitated product using extracts from either the parental (P) line or ago3-25 (M). At-AGO1 antibody was utilized as an IP negative control. (c) Differential localisation of Cr-AGO2 and 3. Total protein isolated from cytoplasmic, total and nuclear fractions are indicated as C, T and N, respectively. Extracts from either Parental or ago3-25 strain are labelled P and M, respectively. Protein bands corresponding to AGO2/3 or Histone 3 (H3) were indicated.

Additional information

Additional information Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

Background

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Product citations

Selected references Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.
Chung et al. (2019) Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression. Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x.

immunogen:	KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)
Host:	Chicken
Clonality:	Polyclonal
Purity:	Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Format:	Liquid
Quantity:	50 µl
storage:	Store at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 2500 (WB)
calculated \| apparent molecular mass [kDa]:	15 \| 17 kDa
Confirmed reactivity:	Arabidopsis thaliana, Chlamydomonas reinhardtii, Lycopersicum esculentum, Triticum aestivum
predicted reactivity:	Arabidopsis thaliana, Brassica napus, Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Hordeum vulgare, Medicago sativa, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pinus pinaster, Pisum sativum, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Triticum aestivum, Zea mays, Vicia faba, Vitis vinifera, Volvox sp. Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example From left to right: 2 wells of nuclear + cytoplasmic enriched protein, 2 wells of total proteins of Chlamydomonas reinhardtii protein saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (AS11 1827, Goat anti-Chicken IgY (H&L), DyLight® 800 conjugated, Agrisera) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner. Courtesy of Dr. Betty Chung, University of Cambridge, United Kingdom
More images:	Reactant: Chlamydomonas reinhardtii (Green Alga) Application: Western Blotting Pudmed ID: 31366981 Journal: Sci Rep Figure Number: 2C Published Date: 2019-07-31 First Author: Chung, B. Y. W., Valli, A., et al. Impact Factor: 4.13 Open Publication Expression and differential localisation of AGO2 and AGO3. (a) Antigen sites utilised for peptide-based antibody production. (b) Western blots for total and ?-2/3-PAZ-immunoprecipitated product using extracts from either the parental (P) line or ago3-25 (M). At-AGO1 antibody was utilized as an IP negative control. (c) Differential localisation of Cr-AGO2 and 3. Total protein isolated from cytoplasmic, total and nuclear fractions are indicated as C, T and N, respectively. Extracts from either Parental or ago3-25 strain are labelled P and M, respectively. Protein bands corresponding to AGO2/3 or Histone 3 (H3) were indicated.
additional information:	Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii
background:	Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
All references:	Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775. Chung et al. (2019) Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression. Sci Rep. 2019 Jul 31;9(1):11091. doi: 10.1038/s41598-019-47415-x.