Name:
Phone:
E-mail:
Address:






Goat anti-Chicken IgY (H&L), DyLightŪ 488 conjugated

AS09 622| Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)

Goat anti-Chicken IgY (H&L), DyLightŪ 488 conjugated in the group Secondary Antibodies / Anti-Chicken  / Fluorescent / DyLightŪ / DyLightŪ 488 at Agrisera AB (Antibodies for research) (AS09 622)
Goat anti-Chicken IgY (H&L), DyLightŪ 488 conjugated



DATA SHEET IN PDF

Qty: 
206
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

Purified Chicken IgY, whole molecule

Host Goat
Clonality Polyclonal
Purity Immunogen affinity purified goat IgG.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Application examples

Immunofluorescence

immunolocalization of plant BiP using hen antibody

BiP localization in 5 days old Arabidopsis thaliana roots. BiP signal shown in green, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Chicken anti-BiP primary antibody was diluted in 1: 1000 and DyLight®488 conjugated goat anti-chicken secondary antibody AS09 622 (green color) was diluted in 1: 1000. Co-staining with DAPI visualized nucleus (blue color).  Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins

No reactivity is observed to: non-immunoglobulin chicken serum proteins

Related products

Background

Background

Goat anti-Chicken IgY (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Chicken IgY (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Chicken IgY

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

Picture (footer):

Immunofluorescence

immunolocalization of plant BiP using hen antibody

BiP localization in 5 days old Arabidopsis thaliana roots. BiP signal shown in green, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Chicken anti-BiP primary antibody was diluted in 1: 1000 and DyLight®488 conjugated goat anti-chicken secondary antibody AS09 622 (green color) was diluted in 1: 1000. Co-staining with DAPI visualized nucleus (blue color).  Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany

additional information:

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins

No reactivity is observed to: non-immunoglobulin chicken serum proteins

Clonality: Polyclonal
Format: Lyophilized
Host: Goat
immunogen:

Purified Chicken IgY, whole molecule

Purity: Immunogen affinity purified goat IgG.
Quantity: 1 mg
recommended dilution: 1 : 20-1 : 2000 for most applications
Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
background:

Goat anti-Chicken IgY (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Chicken IgY (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Chicken IgY

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Related products: Goat anti-Chicken IgY (H&L), DyLightŪ 488 conjugated

AS11 1820 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&L)
206 €
AS16 3264 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY ...
194 €
AS11 1825 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&...
206 €
AS11 1827 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&...
206 €