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Goat anti-Chicken IgY (H&L), DyLightŪ 488 conjugated

AS09 622| Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)

Goat anti-Chicken IgY (H&L), DyLightŪ 488 conjugated in the group Secondary Antibodies / Anti-Chicken  / Fluorescent / DyLightŪ / DyLightŪ 488 at Agrisera AB (Antibodies for research) (AS09 622)

DATA SHEET IN PDF

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195
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Purified Chicken IgY, whole molecule

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Recommended dilution 1 : 20-1 : 2000 for most applications

Reactivity

Application examples

Application examples

Immunofluorescence

immunolocalization of plant BiP using hen antibody

BiP localization in 5 days old Arabidopsis thaliana roots. BiP signal shown in green, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Chicken anti-BiP primary antibody was diluted in 1: 1000 and DyLight®488 conjugated goat anti-chicken secondary antibody AS09 622 (green color) was diluted in 1: 1000. Co-staining with DAPI visualized nucleus (blue color).  Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany

Additional information

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins

No reactivity is observed to: non-immunoglobulin chicken serum proteins

Related products

Background

Background

Goat anti-Chicken IgY (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Chicken IgY (H&L) in immunological assays.

DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Chicken IgY

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

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