Goat anti-Chicken IgY (H&L), DyLight® 650 conjugated

Name: Goat anti-Chicken IgY (H&L), DyLight® 650 conjugated
SKU: AS11 1825
Price: 206 EUR
Availability: InStock

AS11 1825 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)

Goat anti-Chicken IgY (H&L), DyLight® 650 conjugated in the group Secondary Antibodies / Anti-Chicken / Fluorescent / DyLight® / DyLight® 650 at Agrisera AB (Antibodies for research) (AS11 1825)

Product Information

Immunogen

purified chicken IgY, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Recommended dilution 1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)

Reactivity

Confirmed reactivity Chicken IgY heavy and light chains (H&L)

Predicted reactivity Chicken IgY Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

DyLight® 650 (Ex = 652 nm; Em = 672 nm).

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgY and light chains on all chicken immunoglobulins.

No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.

Background

Goat anti-chicken IgY, DyLight® 650 Conjugate is a secondary antibody conjugated to DyLight® 650, which binds to hen immunoglobulins in immunological assays.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Product citations

immunogen:	purified chicken IgY, whole molecule
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications:	Immunocytochemistry (ICC), Immunohistochemistry (IHC)
recommended dilution:	1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Confirmed reactivity:	Chicken IgY heavy and light chains (H&L)
predicted reactivity:	Chicken IgY Heavy and Light chains (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. DyLight® 650 (Ex = 652 nm; Em = 672 nm). Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
additional information (application):	Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgY and light chains on all chicken immunoglobulins. No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
background:	Goat anti-chicken IgY, DyLight® 650 Conjugate is a secondary antibody conjugated to DyLight® 650, which binds to hen immunoglobulins in immunological assays. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

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