H3K9me2 | Histone H3 dimethylated lysine 9
AS16 3194 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, O. sativa , S. lycopersicum

Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Quantity
50 ĩg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Chromatin immunoprecipitation (ChIP), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Human, Solanum lycopersicum
Predicted reactivity
Arabidopsis thaliana, Mouse, Oryza sativa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example

Dot blot analysis to test the cross reactivity of anti-H3K9me2 antibodies with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. 100 to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20 000.

Dot blot analysis to test the cross reactivity of anti-H3K9me2 antibodies with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. 100 to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20 000.
Additional information
Additional information
Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300
Background
Background
Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.
Product citations
Selected references
Liu et al. (2018). Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.
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