showForm Anti-H3K9me2 | Histone H3 dimethylated lysine 9 antibodies

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H3K9me2 | Histone H3 dimethylated lysine 9

AS16 3194 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, O. sativa , S. lycopersicum
H3K9me2 | Histone H3 dimethylated lysine 9  in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3194)

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Product Information

Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS and 0.05% sodium azide
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human, Solanum lycopersicum
Predicted reactivity Arabidopsis thaliana, Mouse, Oryza sativa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

Dot blot using anti-H3K9me2 | Histone H3 dimethylated lysine 9 polyclonal antibodies

Dot blot
analysis to test the cross reactivity of anti-H3K9me2 antibodies with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. 100 to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20 000.

Additional information

Additional information Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300.
Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300.

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collection of antibodies to epigenetics

Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.

Product citations

Selected references Liu et al. (2018). Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.