H3K9me2 | Histone H3 dimethylated lysine 9

Name: H3K9me2 | Histone H3 dimethylated lysine 9
Brand: IKP
SKU: AS16 3194
Price: 393 EUR
Availability: InStock

AS16 3194 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, Human, Mouse, O. sativa , S. lycopersicum

H3K9me2 | Histone H3 dimethylated lysine 9 in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Plant Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3194)

H3K9me2 | Histone H3 dimethylated lysine 9

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum.

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human, Solanum lycopersicum

Predicted reactivity Arabidopsis thaliana, Mouse, Oryza sativa

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Dot blot analysis to test the cross reactivity of anti-H3K9me2 antibodies with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. 100 to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20 000.

Additional information

Additional information Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300

Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300

Background

Background Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.

Product citations

Selected references Liu et al. (2018). Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.

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immunogen:	KLH-conjugated synthetic peptide
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum.
Format:	Liquid
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
recommended dilution:	1-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	15 kDa
Confirmed reactivity:	Human, Solanum lycopersicum
predicted reactivity:	Arabidopsis thaliana, Mouse, Oryza sativa
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Dot blot analysis to test the cross reactivity of anti-H3K9me2 antibodies with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. 100 to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20 000.
additional information:	Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300
additional information (application):	Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300
background:	Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.
All references:	Liu et al. (2018). Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.