H3T3pK4me2 | Histone H3 (dimethylated Lys4, p Thr3)
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Chromatin Immunoprecipitation usingH3T3pK4me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T3pK4me2 and 20 μl of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and normalized to the input chromatin.
Immunofluorescence using anti-H3T3pK4me2 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody was used at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3T3pK4me2 is nuclear and chromosomal. Staining: H3T3pK4me2 is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight® 550 (red).
Western Blot using anti-H3T3pK4me2 antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
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