HCP | Hyper conserved protein
AS10 688 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Prochlorococcus MIT 9313
|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Prochlorococcus MIT 9313|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Whidden et al. (2014). Quantitative and functional characterization of the hyper-conserved protein of prochlorococcus and marine synechococcus. PLoS One. 2014 Oct 31;9(10):e109327. doi: 10.1371/journal.pone.0109327. eCollection 2014.|
7ug of of total protein extract from Prochlorococcus MIT 9313 collected at various days of culture were loaded in each of the samples. The loading scheme is: Lane 1: day 9 sample, Lane 2: day 7 sample, Lane 3: day 9 sample, Lane 4: day 7 sample, Lane 5: day 5 sample, Lane 6: Ladder, Lane 7:240 fmol HCP recombinant standard, Lane 8: 120 fmol recombinant standard, Lane 9: 60 fmol recombinant standard, Lane 10: 30 fmol recombinant standard. The gel was run at 200V for 35 minutes, transferred for 45 minutes at 30V (it was the only blot in the transfer rig). And a nitrocellulose membrane was used for blotting. It was blocked overnight in Blocking Solution. A 1: 4000 dilution of primary anti-HCP antibody was used and the membrane was blocked for an hour. Then the washes in TBS-T were done - 2x briefly, 1x for 15min, and 3x for 5min. A 1:25 000 dilution of secondary antibody was used, and the membrane was blocked for another hour followed by the washes in TBS-T.
It was imaged using ECL Advance detection agents.
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