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HCP | Hyper conserved protein

AS10 688  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Prochlorococcus MIT 9313

HCP | Hyper conserved protein in the group Antibodies for Plant/Algal  / Cyanobacteria at Agrisera AB (Antibodies for research) (AS10 688)

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Product Information

Immunogen

Synechococcus WH8102 recombinant HCP

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

7 kDa

Reactivity

Confirmed reactivity Prochlorococcus MIT 9313
Predicted reactivity Cyanobacteria
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example
western blot using anti-HCP antibody
7ug of of total protein extract from Prochlorococcus MIT 9313 collected at various days of culture were loaded in each of the samples.  The loading scheme is: Lane 1: day 9 sample, Lane 2: day 7 sample, Lane 3: day 9 sample, Lane 4: day 7 sample, Lane 5: day 5 sample, Lane 6: Ladder, Lane 7:240 fmol HCP recombinant standard, Lane 8: 120 fmol recombinant standard, Lane 9: 60 fmol recombinant standard, Lane 10: 30 fmol recombinant standard. The gel was run at 200V for 35 minutes, transferred for 45 minutes at 30V (it was the only blot in the transfer rig).  And a nitrocellulose membrane was used for blotting. It was blocked overnight in Blocking Solution.  A 1: 4000 dilution of primary anti-HCP antibody was used and the membrane was blocked for an hour.  Then the washes in TBS-T were done - 2x briefly, 1x for 15min, and 3x for 5min.  A 1:25 000 dilution of secondary antibody was used, and the membrane was blocked for another hour followed by the washes in TBS-T. Reaction was developed using extreme low femtogram chemiluminescent detection reagent. 

Additional information

Related products

Background

Background

HCP (hyper conserved protein) is perfectly conserved between Prochlorococcus and Synechococcus group. So far its exact function is not known.

Product citations

Selected references Whidden et al. (2014). Quantitative and functional characterization of the hyper-conserved protein of prochlorococcus and marine synechococcus. PLoS One. 2014 Oct 31;9(10):e109327. doi: 10.1371/journal.pone.0109327. eCollection 2014.

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