HCP | Hyper conserved protein
AS10 688 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Prochlorococcus MIT 9313
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Synechococcus WH8102 recombinant HCP
7ug of of total protein extract from Prochlorococcus MIT 9313 collected at various days of culture were loaded in each of the samples. The loading scheme is: Lane 1: day 9 sample, Lane 2: day 7 sample, Lane 3: day 9 sample, Lane 4: day 7 sample, Lane 5: day 5 sample, Lane 6: Ladder, Lane 7:240 fmol HCP recombinant standard, Lane 8: 120 fmol recombinant standard, Lane 9: 60 fmol recombinant standard, Lane 10: 30 fmol recombinant standard. The gel was run at 200V for 35 minutes, transferred for 45 minutes at 30V (it was the only blot in the transfer rig). And a nitrocellulose membrane was used for blotting. It was blocked overnight in Blocking Solution. A 1: 4000 dilution of primary anti-HCP antibody was used and the membrane was blocked for an hour. Then the washes in TBS-T were done - 2x briefly, 1x for 15min, and 3x for 5min. A 1:25 000 dilution of secondary antibody was used, and the membrane was blocked for another hour followed by the washes in TBS-T. Reaction was developed using extreme low femtogram chemiluminescent detection reagent.
HCP (hyper conserved protein) is perfectly conserved between Prochlorococcus and Synechococcus group. So far its exact function is not known.
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