HCP | Hyper conserved protein

Product no: AS10 688

AS10 688  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Prochlorococcus MIT 9313

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  • Product Info
  • Immunogen:

    Synechococcus WH8102 recombinant HCP

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    7 kDa

  • Reactivity
  • Confirmed reactivity: Prochlorococcus MIT 9313
    Predicted reactivity: Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example
    western blot using anti-HCP antibody
    7ug of of total protein extract from Prochlorococcus MIT 9313 collected at various days of culture were loaded in each of the samples.  The loading scheme is: Lane 1: day 9 sample, Lane 2: day 7 sample, Lane 3: day 9 sample, Lane 4: day 7 sample, Lane 5: day 5 sample, Lane 6: Ladder, Lane 7:240 fmol HCP recombinant standard, Lane 8: 120 fmol recombinant standard, Lane 9: 60 fmol recombinant standard, Lane 10: 30 fmol recombinant standard. The gel was run at 200V for 35 minutes, transferred for 45 minutes at 30V (it was the only blot in the transfer rig).  And a nitrocellulose membrane was used for blotting. It was blocked overnight in Blocking Solution.  A 1: 4000 dilution of primary anti-HCP antibody was used and the membrane was blocked for an hour.  Then the washes in TBS-T were done - 2x briefly, 1x for 15min, and 3x for 5min.  A 1:25 000 dilution of secondary antibody was used, and the membrane was blocked for another hour followed by the washes in TBS-T. Reaction was developed using extreme low femtogram chemiluminescent detection reagent. 
  • Background
  • Background:

    HCP (hyper conserved protein) is perfectly conserved between Prochlorococcus and Synechococcus group. So far its exact function is not known.

  • Product Citations
  • Selected references: Whidden et al. (2014). Quantitative and functional characterization of the hyper-conserved protein of prochlorococcus and marine synechococcus. PLoS One. 2014 Oct 31;9(10):e109327. doi: 10.1371/journal.pone.0109327. eCollection 2014.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 16 €
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