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HDA6 | Histone deacetylase 6

AS14 2787 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

HDA6 | Histone deacetylase 6 in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS14 2787)
HDA6 | Histone deacetylase 6



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Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from HDA6 protein of Arabidopsis thaliana Uniprot: Q9FML2, TAIR: At5g63110. Peptide used for immunization is not conserved in HDA9 of Arabidopsis thaliana.
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Chromatin Immunoprecipitation (ChIP), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

52 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica rapa, Capsella rubella, Citrus clementina, Chlamydomonas reinhardii, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Populus trichocarpa, Physcomitrium patens, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is also recognizing recombinant HDA6 overexpressed with GST or MBP in western blot.

ChIP was done according to Saleh et al. (2008).

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Background

Background

HDA6 (histone deacetylase 6) is an enzyme (EC=3.5.1.98) responsible for deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Involved in transcriptional regulation, cell cycle progression and developmental events. Not detected in leaves, stems, flowers and young siliques. Induced by jasmonic acid and ethylene.

Product citations

immunogen:

KLH-conjugated synthetic peptide chosen from HDA6 protein of Arabidopsis thaliana Uniprot: Q9FML2, TAIR: At5g63110. Peptide used for immunization is not conserved in HDA9 of Arabidopsis thaliana.
Reconstitution: For reconstitution add 50 µl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Quantity: 50 ĩg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Chromatin Immunoprecipitation (ChIP), Western blot (WB)
recommended dilution: 1 : 1000 (WB)
calculated | apparent molecular mass [kDa]:

52 kDa
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Brassica rapa, Capsella rubella, Citrus clementina, Chlamydomonas reinhardii, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Populus trichocarpa, Physcomitrium patens, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information (application):

This antibody is also recognizing recombinant HDA6 overexpressed with GST or MBP in western blot.

ChIP was done according to Saleh et al. (2008).
background:

HDA6 (histone deacetylase 6) is an enzyme (EC=3.5.1.98) responsible for deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Involved in transcriptional regulation, cell cycle progression and developmental events. Not detected in leaves, stems, flowers and young siliques. Induced by jasmonic acid and ethylene.

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