HemC | Porphobilinogen-Deaminase (PBG)

Product no: AS08 375

AS08 375 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Thermosynechoccus elongatus

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  • Product Info
  • Immunogen:

    Overexpressed Thermosynechococcus elongatus HemC, UniProt: Q8DIE4

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 µl
    Reconstitution: For reconstitution add 200 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000-1 : 5000 (WB)
    Expected | apparent MW: 34.7 kDa
  • Reactivity
  • Confirmed reactivity: Thermosynechococcus elongatus
    Predicted reactivity: Cyanobacteria

    Species of your interest not listed? Contact us
    Not reactive in:

    Synechococcus sp. PCC 6803

  • Application Examples
  • Application example

    wesern blot detection using anti-PBG (HemC) antibodies

    0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min with chemiluminescent detection reagent and image of the blot was obtained using Fuji LAS 4000.

    Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon, Czech Republic

  • Background
  • Background:

    PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

  • Protocols
  • Antibody protocols
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