HemC | Porphobilinogen-Deaminase (PBG)
AS08 375 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Thermosynechoccus elongatus
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overexpressed Thermosynechococcus elongatus HemC UniProt: Q8DIE4
Synechococcus sp. PCC 6803
0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min with chemiluminescent detection reagent and image of the blot was obtained using Fuji LAS 4000.
Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon, Czech Republic
PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
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