showForm Anti-HemC | porphobilinogen-Deaminase (PGBD) antibodies

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HemC | Porphobilinogen-Deaminase (PBG)

AS08 375 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Thermosynechoccus elongatus

HemC | Porphobilinogen-Deaminase (PBG) in the group Antibodies for Plant/Algal  / Photosynthesis  / Chlorophyll at Agrisera AB (Antibodies for research) (AS08 375)

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Product Information

Immunogen

overexpressed Thermosynechococcus elongatus HemC UniProt: Q8DIE4

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

34.7 kDa

Reactivity

Confirmed reactivity Thermosynechococcus elongatus
Predicted reactivity Cyanobacteria
Not reactive in

Synechococcus sp. PCC 6803

Application examples

Application examples

Application example

wesern blot detection using anti-PBG (HemC) antibodies

0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min with chemiluminescent detection reagent and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon, Czech Republic

Additional information

Related products

Related products

AS07 226 | Anti-HemF, rabbit antibodies

AS07 228 | Anti-HemH | protoporphyrin ferrochelatase, rabbit antibodies

Secondary antibodies

Background

Background

PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

Product citations

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