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Homogalacturonan-1 (clone CCRC-M38)
Product no: AS16 3117-1ml
AS16 3117-1ml | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
From the laboratory of Michael G. Hahn, PhD, University of Georgia
246
€
Delivery:
3-6 business days
- Product Info
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Immunogen: MeBSA-conjugated Arabidopsis thaliana seed mucillage (Hemogalacturonan), non-covalent, Epitope structure for crabohydrate antigen: de-estrified homogalacturonan (DP>4), Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: Cell culture supernatant. Format: Liquid Quantity: 1 ml Storage: Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF) Recommended dilution: 1:10 (IHC)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Homogalacturonan (HG) backbone-1 clade of antibodies and binds to a de-esterified ?-1,4 linked homogalcturonan (HG) epitope (DP>4) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: This antibody recognises fully de-esterified ?-1,4 linked homogalcturonan (HG) epitope with a degree of polymerization (DP) of four or higher (DP>4), Does not recognize a homogalacturonan trimer Additional information (application): This antibody binds to a de-esterified ?-1,4 linked homogalcturonan epitope with a degree of polymerizaion of 4 or more, It does not bind to a homogalacturonan trimer - Background
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Background: Homogalacturonan (HG) is a pectic polysaccharide, composed of α-1,4 linked galacturonic acid residues. Pectin consists of complex net of polysacchari.des which are found in the primary cell walls of terrestial plants and are enriched in non-woody parts. Pectin acts as an extracellular glue which binds cells together. - Product Citations
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Selected references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985. - Reviews:
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