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Homogalacturonan-1 (clone CCRC-M38)

AS16 3117-1ml  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: Arabidopsis thaliana

From the laboratory of Michael G. Hahn, PhD, University of Georgia

Homogalacturonan-1 (clone CCRC-M38) in the group Antibodies Plant/Algal / Cell Wall / Dr. Hahn collection, University of Georgia at Agrisera AB (Antibodies for research) (AS16 3117-1ml)
Homogalacturonan-1 (clone CCRC-M38)



DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen MeBSA-conjugated Arabidopsis thaliana seed mucillage (Hemogalacturonan), non-covalent, Epitope structure for crabohydrate antigen: de-estrified homogalacturonan (DP>4),
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Cell culture supernatant.
Format Liquid
Quantity 1 ml
Storage Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution

1:10 (IHC)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Homogalacturonan (HG) backbone-1 clade of antibodies and binds to a de-esterified ?-1,4 linked homogalcturonan (HG) epitope (DP>4)
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information This antibody recognises fully de-esterified ?-1,4 linked homogalcturonan (HG) epitope with a degree of polymerization (DP) of four or higher (DP>4), Does not recognize a homogalacturonan trimer
This antibody binds to a de-esterified ?-1,4 linked homogalcturonan epitope with a degree of polymerizaion of 4 or more, It does not bind to a homogalacturonan trimer

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Background

Background Homogalacturonan (HG) is a pectic polysaccharide, composed of α-1,4 linked galacturonic acid residues. Pectin consists of complex net of polysacchari.des which are found in the primary cell walls of terrestial plants and are enriched in non-woody parts. Pectin acts as an extracellular glue which binds cells together.

Product citations

Selected references Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen: MeBSA-conjugated Arabidopsis thaliana seed mucillage (Hemogalacturonan), non-covalent, Epitope structure for crabohydrate antigen: de-estrified homogalacturonan (DP>4),
Purity: Cell culture supernatant.
Quantity: 1 ml
recommended dilution:

1:10 (IHC)
storage: Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgG1
tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Homogalacturonan (HG) backbone-1 clade of antibodies and binds to a de-esterified ?-1,4 linked homogalcturonan (HG) epitope (DP>4)
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: This antibody recognises fully de-esterified ?-1,4 linked homogalcturonan (HG) epitope with a degree of polymerization (DP) of four or higher (DP>4), Does not recognize a homogalacturonan trimer
additional information (application): This antibody binds to a de-esterified ?-1,4 linked homogalcturonan epitope with a degree of polymerizaion of 4 or more, It does not bind to a homogalacturonan trimer
All references: Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
background: Homogalacturonan (HG) is a pectic polysaccharide, composed of α-1,4 linked galacturonic acid residues. Pectin consists of complex net of polysacchari.des which are found in the primary cell walls of terrestial plants and are enriched in non-woody parts. Pectin acts as an extracellular glue which binds cells together.