Homogalacturonan-1 (clone CCRC-M38)
AS16 3117-1ml | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
From the laboratory of Michael G. Hahn, PhD, University of Georgia

Data sheet | Product citations | Add review |
Product Information
Immunogen
MeBSA-conjugated Arabidopsis thaliana seed mucillage (Hemogalacturonan), non-covalent, Epitope structure for crabohydrate antigen: de-estrified homogalacturonan (DP>4),
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Cell culture supernatant.
Format
Liquid
Quantity
1 ml
Storage
Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Recommended dilution
1:10 (IHC)
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Homogalacturonan (HG) backbone-1 clade of antibodies and binds to a de-esterified ?-1,4 linked homogalcturonan (HG) epitope (DP>4)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
This antibody recognises fully de-esterified ?-1,4 linked homogalcturonan (HG) epitope with a degree of polymerization (DP) of four or higher (DP>4), Does not recognize a homogalacturonan trimer
This antibody binds to a de-esterified ?-1,4 linked homogalcturonan epitope with a degree of polymerizaion of 4 or more, It does not bind to a homogalacturonan trimer
Background
Background
Homogalacturonan (HG) is a pectic polysaccharide, composed of α-1,4 linked galacturonic acid residues. Pectin consists of complex net of polysacchari.des which are found in the primary cell walls of terrestial plants and are enriched in non-woody parts. Pectin acts as an extracellular glue which binds cells together.
Product citations
Selected references
Pattathil et al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
Patathil et al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.