HSP90-2 | heat shock protein 90-2

AS11 1629 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Sorghum bicolor

Product Information

Immunogen

Full length recombinant HSP90-2 of Arabidopsis thaliana, UniProt: F4K6B6-1, TAIR:

AT5G56030

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW

80.6 | 95 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Sorghum bicolor

Predicted reactivity Glycne max, Hordeum vulgare, Micromonas pulsilla, Nicotiana benthamina, Nicotiana tabacum, Ostreococcus lucimarinus, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Reactant: Solanum tuberosum (Potato)

Application: Western Blotting

Pudmed ID: 26612975

Journal: Mol Breed

Figure Number: 3B

Published Date: 2015-11-28

First Author: So?tys-Kalina, D., Szajko, K., et al.

Impact Factor: 2.533

Open Publication

a Expression level of the Hsp90 gene in tubers after cold storage (CS); ns non-significant differences between parental clones (Student’s t test); a and b homogenous groups between bulk samples LII and DII (Tukey’s test). b Amounts of Hsp90-1 and Hsp90-2 protein isoforms in tubers after cold storage (CS); Marker—Prosieve® QuadColor™ Protein Marker. Parental clones: DG 97-952 (chip color 7) and DG 08-26/39 (chip color 4); bulk samples: LII (chip color 8–9) and DII (chip color 1–3)

Additional information

Additional information Antibody reacts more with constitutive isofrom Hsp90-2 than heat inducible Hsp90-1

This product can be sold containing ProClin if requested

Related products

collection of antibodies to plant HSP proteins

Plant protein extraction buffer

Secondary antibodies

Background

HSP90-2 (heat shock protein 90-2) is a constitutive isoform of HSP90, expressed in all tissues and abundant in root apical meristem, pollen and tapetum.

Alternative names: ATHSP90.2, EARLY-RESPONSIVE TO DEHYDRATION 8, ERD8, HEAT SHOCK PROTEIN 90.2, HEAT SHOCK PROTEIN 81-2, HEAT SHOCK PROTEIN 81.2, HEAT SHOCK PROTEIN 90.2, HSP81-2, HSP81.2, HSP90.2.

Product citations

Selected references Gao et al. (2021) Identification of a bacterial-type ATP-binding cassette transporter implicated in aluminum tolerance in sweet sorghum (Sorghum bicolor L.). Plant Signal Behav. 2021 Jul 3;16(7):1916211. doi: 10.1080/15592324.2021.1916211. Epub 2021 May 26. PMID: 34034635; PMCID: PMC8205057.
Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.
He et a. (2012).SpecificMissenseAlleles of theArabidopsisJasmonicAcidCo-ReceptorCOI1RegulateInnateImmuneReceptorAccumulation andFunction. PloS Genetics, Open Access.