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Anti-ICE1 | Inducer of CBF expression 1
Product no: AS16 3971
AS16 3971 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
329
€
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- Data sheet
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICE1 sequence, UniProt: Q9LSE2#Q9LSE2-2, TAIR: AT3G26744. The peptide is not conserved in protein coded by SCRM2 gene. UniProt: A0A1P8AMV9
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB)
Expected | apparent MW: 43 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana
Predicted reactivity: Brassica napus, Brassica rapa, Capsella bursa-pastoris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens
Species of your interest not listed? Contact usNot reactive in: Solanum lycopersicum - Background
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Background: ICE1 (Inducer of CBF expression 1) is a negative regulator in brassinosteroid signal transduction pathway important for plant growth. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates BHLH150, beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues. Upon brassinosteroid signaling, inhibits stomatal development by phosphorylating and inhibiting the MAPKK kinase YDA and the MAPK kinases MKK4 and MKK5. - Product Citations
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Selected references: Wu et al. (2024). Brassica rapa BrICE1 and BrICE2 Positively Regulate the Cold Tolerance via CBF and ROS Pathways, Balancing Growth and Defense in Transgenic Arabidopsis. Plants (Basel) . 2024 Sep 20;13(18):2625. doi: 10.3390/plants13182625.
Patir-Nebioglu et al. (2019). Pyrophosphate modulates plant stress responses via SUMOylation. Elife. 2019 Feb 20;8. pii: e44213. doi: 10.7554/eLife.44213. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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