Anti-ICE1 | Inducer of CBF expression 1

Product no: AS16 3971

AS16 3971 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Data sheet
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICE1 sequence, UniProt: Q9LSE2#Q9LSE2-2, TAIR: AT3G26744. The peptide is not conserved in protein coded by SCRM2 gene. UniProt: A0A1P8AMV9

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution:

    1 : 1000 (WB)

    Expected | apparent MW: 43 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica napus, Brassica rapa, Capsella bursa-pastoris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens
    Species of your interest not listed? Contact us
    Not reactive in: Solanum lycopersicum
  • Background
  • Background: ICE1 (Inducer of CBF expression 1)  is a negative regulator in brassinosteroid signal transduction pathway important for plant growth. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates BHLH150, beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues. Upon brassinosteroid signaling, inhibits stomatal development by phosphorylating and inhibiting the MAPKK kinase YDA and the MAPK kinases MKK4 and MKK5.
  • Product Citations
  • Selected references: Patir-Nebioglu et al. (2019). Pyrophosphate modulates plant stress responses via SUMOylation. Elife. 2019 Feb 20;8. pii: e44213. doi: 10.7554/eLife.44213.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

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AS16 ECL-S | extreme low femtogram detection  |  Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

Choose the appropriate volume in the drop down menu to the right

From 17 €
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