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ICE1 | Inducer of CBF expression 1

AS16 3971 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

ICE1 | Inducer of CBF expression 1 in the group Antibodies for Plant/Algal  / Environmental Stress / Salt stress at Agrisera AB (Antibodies for research) (AS16 3971)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICE1 sequence, UniProt: Q9LSE2#Q9LSE2-2, TAIR: AT3G26744. The peptide is not conserved in protein coded by SCRM2 gene. UniProt: A0A1P8AMV9

Host Rabbit
Clonality Polyclonal
Purity Affinity purfiied serum in PBS, pH 7.4.
Format Lyophilized
Quantity

50 µg

Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution

1 : 1000 (WB)

Expected | apparent MW 43 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Brassica rapa, Capsella bursa-pastoris, Capsella rubella, Eutrema salsugineum, Noccaea caerulescens
Species of your interest not listed? Contact us
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Related products

Background

Background ICE1 (Inducer of CBF expression 1)  is a negative regulator in brassinosteroid signal transduction pathway important for plant growth. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates BHLH150, beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues. Upon brassinosteroid signaling, inhibits stomatal development by phosphorylating and inhibiting the MAPKK kinase YDA and the MAPK kinases MKK4 and MKK5.

Product citations

Selected references Patir-Nebioglu et al. (2019). Pyrophosphate modulates plant stress responses via SUMOylation. Elife. 2019 Feb 20;8. pii: e44213. doi: 10.7554/eLife.44213.

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