IFN alpha | Interferon alpha

Product no: AS15 2869

AS15 2869 | Clonality: Polyclonal | Host: Goat | Reactivity: Human 

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  • Product Info
  • Immunogen: Recombinant IFN-alpha88, produced in E.coli
    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified in a carbonate buffer pH 8-8.5 with NaCl and 0.02 % sodium azide.
    Format: Lyophilized
    Quantity: 100 g
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 0.2 µg/ml (WB)
    Expected | apparent MW:

    19-27 kDa (in reduced conditions)

  • Reactivity
  • Confirmed reactivity: Human
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using goat anti-interferon alpha antibodies on various subtypes of interferon alpha

    Following subtypes of interferon alpha were analyzed: Multiferon control RC1317 (1), Prestained LMW (2), Alpha A (2a) (3), Alpha B2 (8) (4), Alpha C (10) (5), Alpha D (1) (6), Alpha F (21) (7), Alpha G (5) (8), Alpha H2 (14) (9), Alpha I (17) (10), Alpha J1 (7) (11), Alpha K (6) (12), Alpha 4b (4) (13), Alpha WA (16) (14)
    All HuIFN alpha subtypes are recognised by this antibody. Detection of interferon alpha-17 was very weak but still detectable showing that the antibody recognizes the subtype but probably with a lower affinity.
  • Additional Information
  • Additional information: Antibodies are purified on a leukocyte IFN affinity column and resulting purity is >95 %, analyzed by coomassie stained SDS-PAGE gels.

    The antibodies react with 12 different commercially available recombinant subtypes of human IFN-alpha, with lowest reactivity to interferon alpha-17.
  • Background
  • Background: IFN alpha (interferon alpha) is produced by macrophages and have antiviral activities. It stimulated production of protein kinase and an oligoadenulate synthetase.
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