IFN alpha | Interferon alpha

AS15 2869 | Clonality: Polyclonal | Host: Goat | Reactivity: Human

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Product Information

Immunogen Recombinant IFN-alpha88, produced in E.coli

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified in a carbonate buffer pH 8-8.5 with NaCl and 0.02 % sodium azide.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 0.2 µg/ml (WB)

Expected | apparent MW

19-27 kDa (in reduced conditions)

Reactivity

Confirmed reactivity Human

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

Following subtypes of interferon alpha were analyzed: Multiferon control RC1317 (1), Prestained LMW (2), Alpha A (2a) (3), Alpha B2 (8) (4), Alpha C (10) (5), Alpha D (1) (6), Alpha F (21) (7), Alpha G (5) (8), Alpha H2 (14) (9), Alpha I (17) (10), Alpha J1 (7) (11), Alpha K (6) (12), Alpha 4b (4) (13), Alpha WA (16) (14).
All HuIFN alpha subtypes are recognised by this antibody. Detection of interferon alpha-17 was very weak but still detectable showing that the antibody recognizes the subtype but probably with a lower affinity.

Additional information

Additional information Antibodies are purified on a leukocyte IFN affinity column and resulting purity is >95 %, analyzed by coomassie stained SDS-PAGE gels.

The antibodies react with 12 different commercially available recombinant subtypes of human IFN-alpha, with lowest reactivity to interferon alpha-17.

Background

Background IFN alpha (interferon alpha) is produced by macrophages and have antiviral activities. It stimulated production of protein kinase and an oligoadenulate synthetase.

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Confirmed reactivity:	Human
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	IFN alpha (interferon alpha) is produced by macrophages and have antiviral activities. It stimulated production of protein kinase and an oligoadenulate synthetase.
additional information:	Antibodies are purified on a leukocyte IFN affinity column and resulting purity is >95 %, analyzed by coomassie stained SDS-PAGE gels. The antibodies react with 12 different commercially available recombinant subtypes of human IFN-alpha, with lowest reactivity to interferon alpha-17.
Picture (footer):	application example Following subtypes of interferon alpha were analyzed: Multiferon control RC1317 (1), Prestained LMW (2), Alpha A (2a) (3), Alpha B2 (8) (4), Alpha C (10) (5), Alpha D (1) (6), Alpha F (21) (7), Alpha G (5) (8), Alpha H2 (14) (9), Alpha I (17) (10), Alpha J1 (7) (11), Alpha K (6) (12), Alpha 4b (4) (13), Alpha WA (16) (14). All HuIFN alpha subtypes are recognised by this antibody. Detection of interferon alpha-17 was very weak but still detectable showing that the antibody recognizes the subtype but probably with a lower affinity.
calculated \| apparent molecular mass [kDa]:	19-27 kDa (in reduced conditions)
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	Recombinant IFN-alpha88, produced in E.coli
Purity:	Immunogen affinity purified in a carbonate buffer pH 8-8.5 with NaCl and 0.02 % sodium azide.
Quantity:	100 µg
recommended dilution:	0.2 µg/ml (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)