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IFN alpha | Interferon alpha

AS15 2869 | Clonality: Polyclonal | Host: Goat | Reactivity: Human 

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IFN alpha | Interferon alpha in the group Antibodies for Human/Animal  / Human Proteins / Immunology at Agrisera AB (Antibodies for research) (AS15 2869)

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Product Information

Immunogen Recombinant IFN-alpha88, produced in E.coli
Host Goat
Clonality Polyclonal
Purity Affinity purified
Format Lyophilized in a carbonate buffer with NaCl and 0.02 % sodium azide, pH 8.0-8.5
Quantity 100 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 0.2 µg/ml (WB)
Expected | apparent MW

19-27 kDa (in reduced conditions)

Reactivity

Confirmed reactivity Human
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using goat anti-interferon alpha antibodies on various subtypes of interferon alpha

Following subtypes of interferon alpha were analyzed: Multiferon control RC1317 (1), Prestained LMW (2), Alpha A (2a) (3), Alpha B2 (8) (4), Alpha C (10) (5), Alpha D (1) (6), Alpha F (21) (7), Alpha G (5) (8), Alpha H2 (14) (9), Alpha I (17) (10), Alpha J1 (7) (11), Alpha K (6) (12), Alpha 4b (4) (13), Alpha WA (16) (14)
All HuIFN alpha subtypes are recognised by this antibody. Detection of interferon alpha-17 was very weak but still detectable showing that the antibody recognizes the subtype but probably with a lower affinity.

Additional information

Additional information Antibodies are purified on a leukocyte IFN affinity column and resulting purity is >95 %, analyzed by coomassie stained SDS-PAGE gels.

The antibodies react with 12 different commercially available recombinant subtypes of human IFN-alpha, with lowest reactivity to interferon alpha-17.

Related products

Related products IMS01-145-319 | Anti-IFNG | Interferon gamma, chicken antibodies

Background

Background IFN alpha (interferon alpha) is produced by macrophages and have antiviral activities. It stimulated production of protein kinase and an oligoadenulate synthetase.

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