IFN alpha | Interferon alpha
AS15 2869 | Clonality: Polyclonal | Host: Goat | Reactivity: Human
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Product Information
Immunogen
Recombinant IFN-alpha88, produced in E.coli
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified in a carbonate buffer pH 8-8.5 with NaCl and 0.02 % sodium azide.
Format
Lyophilized
Quantity
100 ĩg
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
0.2 µg/ml (WB)
Expected | apparent MW
19-27 kDa (in reduced conditions)
Reactivity
Confirmed reactivity
Human
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example

Following subtypes of interferon alpha were analyzed: Multiferon control RC1317 (1), Prestained LMW (2), Alpha A (2a) (3), Alpha B2 (8) (4), Alpha C (10) (5), Alpha D (1) (6), Alpha F (21) (7), Alpha G (5) (8), Alpha H2 (14) (9), Alpha I (17) (10), Alpha J1 (7) (11), Alpha K (6) (12), Alpha 4b (4) (13), Alpha WA (16) (14).
All HuIFN alpha subtypes are recognised by this antibody. Detection of interferon alpha-17 was very weak but still detectable showing that the antibody recognizes the subtype but probably with a lower affinity.

Following subtypes of interferon alpha were analyzed: Multiferon control RC1317 (1), Prestained LMW (2), Alpha A (2a) (3), Alpha B2 (8) (4), Alpha C (10) (5), Alpha D (1) (6), Alpha F (21) (7), Alpha G (5) (8), Alpha H2 (14) (9), Alpha I (17) (10), Alpha J1 (7) (11), Alpha K (6) (12), Alpha 4b (4) (13), Alpha WA (16) (14).
All HuIFN alpha subtypes are recognised by this antibody. Detection of interferon alpha-17 was very weak but still detectable showing that the antibody recognizes the subtype but probably with a lower affinity.
Additional information
Additional information
Antibodies are purified on a leukocyte IFN affinity column and resulting purity is >95 %, analyzed by coomassie stained SDS-PAGE gels.
The antibodies react with 12 different commercially available recombinant subtypes of human IFN-alpha, with lowest reactivity to interferon alpha-17.
The antibodies react with 12 different commercially available recombinant subtypes of human IFN-alpha, with lowest reactivity to interferon alpha-17.
Background
Background
IFN alpha (interferon alpha) is produced by macrophages and have antiviral activities. It stimulated production of protein kinase and an oligoadenulate synthetase.
Product citations
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