Rabbit anti-Goat IgG (H&L), ALP conjugated

Name: Rabbit anti-Goat IgG (H&L), ALP conjugated
SKU: AS09 630
Price: 246 EUR
Availability: InStock

AS09 630 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Goat IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Rabbit anti-Goat IgG (H&L), ALP conjugated in the group Secondary Antibodies / Anti-Goat / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS09 630)

Product Information

Immunogen

Purified Goat IgG, whole molecule

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified rabbit IgG.

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Goat IgG heavy and light chains (H&L)

Predicted reactivity Goat IgG Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all goat immunoglobulins in immunological assays.

Product citations

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immunogen:	Purified Goat IgG, whole molecule
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified rabbit IgG.
Format:	Liquid
Quantity:	1 mg
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Confirmed reactivity:	Goat IgG heavy and light chains (H&L)
predicted reactivity:	Goat IgG Heavy and Light chains (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
additional information (application):	No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
background:	Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all goat immunoglobulins in immunological assays.