Rabbit anti-Goat IgG Fc, HRP conjugated

Name: Rabbit anti-Goat IgG Fc, HRP conjugated
SKU: AS10 1003
Price: 246 EUR
Availability: InStock

AS10 1003 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Goat IgG Fc

10 % until end of 2023. Use discount code: Conj10

Rabbit anti-Goat IgG Fc, HRP conjugated in the group Secondary Antibodies / Anti-Goat / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 1003)

Product Information

Host Rabbit

Clonality Polyclonal

Purity Antien Immunogen affinity purified rabbit IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Recommended dilution 1 : 500-1 : 5000 (IHC),1 : 10 000-1 : 50 000 (WB)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.

Minimum cross-reactivity is observed to the light chains or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Background Rabbit anti-goat IgG Fc is a secondary antibody to goat Fc part conjugated to HRP. Antibodies are affinity purified using solid phase goat IgG.

Product citations

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Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Antien Immunogen affinity purified rabbit IgG.
Format:	Lyophilized
Quantity:	1 mg
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
recommended dilution:	1 : 500-1 : 5000 (IHC),1 : 10 000-1 : 50 000 (WB)
additional information:	This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis. Minimum cross-reactivity is observed to the light chains or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
background:	Rabbit anti-goat IgG Fc is a secondary antibody to goat Fc part conjugated to HRP. Antibodies are affinity purified using solid phase goat IgG.