Rabbit anti-Goat IgG (H&L), FITC conjugated

AS10 1155 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Goat IgG (H&L)

Product Information

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified IgG.

Format Liquid

Quantity 2 mg

Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.

Recommended dilution 1 : 50-1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.

Background

Background Rabbit anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase goat IgG.

Product citations

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Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
Purity:	Immunogen affinity purified IgG.
Quantity:	2 mg
recommended dilution:	1 : 50-1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)
storage:	Store at 2-8°C. Shelf life of this product is one year from date of receipt.
additional information:	This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.
background:	Rabbit anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase goat IgG.