IFR | isoflavone reductase
AS16 3847 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa
Limited stock.
Limited stock.
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 100 ĩl of sterile water
Storage
The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
Tested applications
Western blot (WB)
Recommended dilution
1 : 500 (WB)
Expected | apparent MW
33 | 40 kDa
Reactivity
Confirmed reactivity
Oryza sativa
Predicted reactivity
Oryza sativa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
50-100 µg of total protein from Oryza sativa cv. 9311 flag leaf at maturing stage, extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10-20 seconds.
50-100 µg of total protein from Oryza sativa cv. 9311 flag leaf at maturing stage, extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10-20 seconds.
Additional information
Background
Background
IFR (isoflavone reductase) is an enzyme involved in isoflavonoid biosynthesis in plants.
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