IKP

IFR | isoflavone reductase

Product no: AS16 3847
AS16 3847 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa

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  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from Oryza sativa UniProt: Q9FTN5, LOC_Os01g01660.1

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: The antibody may be stored at -20C for one year in its original formulation. Additionally, antibody may be stored at 2C to 8C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 500 (WB)
    Expected | apparent MW: 33 | 40 kDa
  • Reactivity
  • Confirmed reactivity: Oryza sativa
    Predicted reactivity: Oryza sativa
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example 

    western blot using anti-IFR, isoflavone reductase antibodies
    50-100 µg of total protein from Oryza sativa cv. 9311 flag leaf at maturing stage, extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10-20 seconds.
  • Background
  • Background: IFR (isoflavone reductase) is an enzyme involved in isoflavonoid biosynthesis in plants.
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