LEA6-3 | late embryogenesis abundant protein 6-3

Recombinant PvLEA 6 (original name LEA18) (AF240774.1, gi 8895962) from Phaseolus vulgaris, a group 6 LEA protein (Battaglia et al., 2008)

8.7 | 14 kDa

application example


Recombiant PvLEA6-3 was separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer for 1 h. Blots were blocked ON at 4ºC in 2% non-fat milk with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 2% non-fat milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 min.
Recombinant AtLEA4-5 was used as a negative control.

Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico

AS13 2758 | Anti-LEA4-5 | Late embryogenesis abundant protein 4-5, rabbit antibodies

Plant protein extraction buffer

LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress.

Synonymes: Putative late embryogenesis abundant protein LEA.