Name:
Phone:
E-mail:
Message


 


LEA6-3 | late embryogenesis abundant protein 6-3

AS13 2756 | Clonality: Polyclonal |  Host: Rabbit |  Reactivity: Phaseolus vulgaris recombiant LEA6-3

LEA6-3 | late embryogenesis abundant protein 6-3 in the group Antibodies for Plant/Algal  / Environmental Stress / Drought stress at Agrisera AB (Antibodies for research) (AS13 2756)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

Recombinant PvLEA 6 (original name LEA18) (AF240774.1, gi 8895962) from Phaseolus vulgaris, a group 6 LEA protein (Battaglia et al., 2008)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

8.7 | 14 kDa

Reactivity

Confirmed reactivity Recombiant PvLEA6-3
Predicted reactivity Arabidopsis thaliana, Arachis hypogaea, Glycine max, Medicago truncatula, Phaseolus vulgaris
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using anti-LEA6 antibodies
Recombiant PvLEA6-3 was separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer for 1 h. Blots were blocked ON at 4ºC in 2% non-fat milk with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 2% non-fat milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 min.
Recombinant AtLEA4-5 was used as a negative control.

Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico


Additional information

Related products

Related products

AS13 2758 | Anti-LEA4-5 | Late embryogenesis abundant protein 4-5, rabbit antibodies

Plant protein extraction buffer

Background

Background

LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress.

Synonymes: Putative late embryogenesis abundant protein LEA.

Product citations

Related products: LEA6-3 | late embryogenesis abundant protein 6-3

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection...
From 15 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
...
190 €
AS13 2758A | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana...
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items