LEA4-5 | Late embryogenesis abundant protein 4-5 (affinity purified)

272 €
Buy 2 items of this product for 202 €/items
Buy 3 items of this product for 185 €/items

AS13 2758A | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana


34 st
Item No:
AS13 2758A

Info: More information Product suggestions Add review
product information

LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.


recombinant LEA4-5 from Arabidopsis thaliana: UniProt: Q9FG31, TAIR: AT5G06760, a group 4 LEA protein (Battaglia et al., 2008)

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS13 2758 | Anti-LEA4-5 | Late embryogenesis abundant protein 4-5, rabbit antibody
AS13 2756 | Anti-LEA6-3 | Late embryogenesis abundant protein 6-3, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

16 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arachis hypogaea, Brassica sp., Glycine max, Medicago truncatula, Phaseolus vulgaris, Thellungiella halophila
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in August 2016.

application information

western blot using affinity purified anti-AtLEA4-5 antibodies

Protein extracts were obtained from Arabidopsis thaliana seeds (extract), beside recombinant AtLEA4-5 loaded in indicated amounts. Following extraction buffer was used: 0.7 M sucrose, 0.5 M Tris-base, 30 mM HCl, 50 mM EDTA, 0.1 M KCl, 2% β-mercaptoethanol, 12 mg/ml poly-vinyl-poly-pyrrolidone (PVPP). This buffer was complemented with 2mL of equilbrated phenol before extraction. Samples were centrifuged and the protein phase was recovered; the extracted proteins were precipitated with 0.1 m ammonium acetate dissolved in methanol, centrifuged and the pellet washed with cold (-20°C) 80% acetone. The protein pellet was dissolved in SDS-solubilization buffer (1% CHES, 2% SDS, 2% ß-mercaptoethanol, 10% glycerol). Thirty µg of seed protein extract was denatured with Laemmli buffer at 95 °C for 5 min and proteins were separated on 15% SDS-PAGE and blotted 1.5 h to nitrocellulose membrane in a liquid transfer system. Blots were blocked with 2% skim milk ON at 4°C with agitation. After rinsing with TBS, blots were incubated in the primary antibody at a dilution of 1:1 000 (anti-AtLEA4-5) for 3h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed once for 15 min and 3 times for 5 min in TBS-T at 4°C with agitation. Then, blots were incubated in secondary antibody (anti-rabbit IgG horse-radish peroxidase conjugate) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with SuperSignal West Pico Chemiluminescent Substrate, Thermo Fisher Scientific. Exposure time was for 30 seconds.

Courtesy of Dr. Alejandra Covarrubias, UNAM, Mexico Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at