Llama anti-Rabbit IgG (H&L), DyLight® 488 conjugated

AS15 2839 | Clonality: Polyclonal | Host: Llama | Reactivity: Rabbit IgG (H&L)


product information
Background		Llama anti-Rabbit IgG (H&L), DyLight 488 Conjugate is a secondary antibody which binds to heavy (γ) chains chains on rabbit IgG light chains on all rabbit immunoglobulins. DyLight® 488 Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L). DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Immunogen		Purified Rabbit IgG, whole molecule
Host		Llama
Clonality		Polyclonal
Clone
Purity		Affinity purified llama IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		Immunoassays
Related products		Other DyLight® anti-Rabbit secondary antibodies
Additional information		10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, 0.05 % (w/v) of Sodium azide as preservative. Based on IEP, this antibody reacts with: heavy (γ) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins

application information
Recommended dilution		The optimal working dilution should be determined by the investigator.
Expected \| apparent MW
Confirmed reactivity		reacts with: heavy (γ) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins
Predicted reactivity
Not reactive in		Based on IEP, no reactivity is observed to:˖ non-immunoglobulin rabbit serum proteins
Additional information
Selected references		to be added when available

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