Llama anti-Rabbit IgG (H&L), DyLight® 488 conjugated

AS15 2839 | Clonality: Polyclonal | Host: Llama | Reactivity: Rabbit IgG (H&L)

Product Information

Immunogen

Purified Rabbit IgG, whole molecule

Host Llama

Clonality Polyclonal

Purity Immunogen affinity purified rabbit IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications Immunoassays

Recommended dilution The optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity reacts with: heavy (?) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins

Not reactive in

Based on IEP, no reactivity is observed to:˖ non-immunoglobulin rabbit serum proteins

Application examples

Additional information

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, 0.05 % (w/v) of Sodium azide as preservative.

Based on IEP, this antibody reacts with: heavy (γ) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins
Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins

Background

Llama anti-Rabbit IgG (H&L), DyLight 488 Conjugate is a secondary antibody which binds to heavy (γ) chains chains on rabbit IgG light chains on all rabbit immunoglobulins.

DyLight® 488 Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Product citations

additional information:	10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, 0.05 % (w/v) of Sodium azide as preservative. Based on IEP, this antibody reacts with: heavy (γ) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Llama
immunogen:	Purified Rabbit IgG, whole molecule
Purity:	Immunogen affinity purified rabbit IgG.
Quantity:	1 mg
recommended dilution:	The optimal working dilution should be determined by the investigator
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
tested applications:	Immunoassays
background:	Llama anti-Rabbit IgG (H&L), DyLight 488 Conjugate is a secondary antibody which binds to heavy (γ) chains chains on rabbit IgG light chains on all rabbit immunoglobulins. DyLight® 488 Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L). DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Confirmed reactivity:	reacts with: heavy (?) chains chains on rabbit IgG light chains on all on all rabbit immunoglobulins
not reactive in:	Based on IEP, no reactivity is observed to:˖ non-immunoglobulin rabbit serum proteins

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