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NAD-ME | Mitochondrial NAD-dependent malic enzyme

AS16 3932  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Zea mays

NAD-ME | Mitochondrial NAD-dependent malic enzyme in the group Alla produktegenskaper at Agrisera AB (Antibodies for research) (AS16 3932)
NAD-ME | Mitochondrial NAD-dependent malic enzyme



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Product Information

Immunogen Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves, UniProt: P37224
Host Rabbit
Clonality Polyclonal
Purity Serum + 0.01% sodium azide.
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunoprecipiatation (IP), Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 25 to 1 : 100 (IL), 1 : 1000 (WB)
Expected | apparent MW

65 kDa

Reactivity

Confirmed reactivity Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Nicotiana tabacum, Zea mays
Predicted reactivity Amborella trichopoda, Arabis alpina, Beta vulgaris, Brachypodium distachyon, Brassica oleracea, Citrus sinensis, Coffea canephora, Cucumis sativus , Cynara cardunculus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium raimondii, Hordeum vulgare, Jatropha curcas, Kalanchoe fedtschenkoi, Malus domestica, Medicago truncatula, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Picea sitcHensis, Populus trichocarpa, Prunus persica, Ricinus communis, Selaginella moellendorffii, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zostera marina, Wollemia nobilis, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody can be used in immunolocalization using immunogold TEM.

This antibody recognizes the large subunit (MEL) of the NAD-ME.

Immunolocalization method is described in Long et al. (1994).

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Background

Background NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation.

Product citations

Selected references Long et al. (1994). Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria. J Biol Chem. 1994 Jan 28;269(4):2827-33.
immunogen: Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves, UniProt: P37224
Reconstitution: For reconstitution add 50 µl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum + 0.01% sodium azide.
Format: Lyophilized
Quantity: 50 µl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Immunoprecipiatation (IP), Immunolocalization (IL), Western blot (WB)
recommended dilution: 1 : 25 to 1 : 100 (IL), 1 : 1000 (WB)
calculated | apparent molecular mass [kDa]:

65 kDa

Confirmed reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Nicotiana tabacum, Zea mays
predicted reactivity: Amborella trichopoda, Arabis alpina, Beta vulgaris, Brachypodium distachyon, Brassica oleracea, Citrus sinensis, Coffea canephora, Cucumis sativus , Cynara cardunculus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium raimondii, Hordeum vulgare, Jatropha curcas, Kalanchoe fedtschenkoi, Malus domestica, Medicago truncatula, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Picea sitcHensis, Populus trichocarpa, Prunus persica, Ricinus communis, Selaginella moellendorffii, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zostera marina, Wollemia nobilis, Vitis vinifera
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: Antibody can be used in immunolocalization using immunogold TEM.

This antibody recognizes the large subunit (MEL) of the NAD-ME.

Immunolocalization method is described in Long et al. (1994).
background: NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation.
All references: Long et al. (1994). Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria. J Biol Chem. 1994 Jan 28;269(4):2827-33.

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