NAD-ME | Mitochondrial NAD-dependent malic enzyme
AS16 3932 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Zea mays

Data sheet | Product citations | Protocols | Customer reviews |
Product Information
Immunogen
Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves, UniProt: P37224
Host
Rabbit
Clonality
Polyclonal
Purity
Serum + 0.01% sodium azide.
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunoprecipiatation (IP), Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 25 to 1 : 100 (IL), 1 : 1000 (WB)
Expected | apparent MW
65 kDa
Reactivity
Confirmed reactivity
Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Nicotiana tabacum, Zea mays
Predicted reactivity
Amborella trichopoda, Arabis alpina, Beta vulgaris, Brachypodium distachyon, Brassica oleracea, Citrus sinensis, Coffea canephora, Cucumis sativus , Cynara cardunculus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium raimondii, Hordeum vulgare, Jatropha curcas, Kalanchoe fedtschenkoi, Malus domestica, Medicago truncatula, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Physcomitrium patens, Picea sitcHensis, Populus trichocarpa, Prunus persica, Ricinus communis, Selaginella moellendorffii, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zostera marina, Wollemia nobilis, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Antibody can be used in immunolocalization using immunogold TEM.
This antibody recognizes the large subunit (MEL) of the NAD-ME.
Immunolocalization method is described in Long et al. (1994).
This antibody recognizes the large subunit (MEL) of the NAD-ME.
Immunolocalization method is described in Long et al. (1994).
Background
Background
NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation.
Product citations
Selected references
Long et al. (1994). Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria. J Biol Chem. 1994 Jan 28;269(4):2827-33.
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