NBR1 | Autophagy substrate NBR1 (affinity purified)

AS14 2805A | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

Product Information

Immunogen UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 500-1 : 5000 (WB)

Expected | apparent MW

75 | 100 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassicaceae family

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

A specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.

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Background

Background NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin.

Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.