NBR1 | Autophagy substrate NBR1 (affinity purified)

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AS14 2805A | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana


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Item No:
AS14 2805A

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product information
Background NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.
Immunogen UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 500-1 : 5000 (WB)
Expected | apparent MW

75 | 100 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassicaceae family
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

A specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.

Selected references to be added when available, antibody release in February 2015

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