NBR1 | Autophagy substrate NBR1 (affinity purified)

Name: NBR1 | Autophagy substrate NBR1 (affinity purified)
Brand: 1
SKU: AS14 2805A
Price: 302 EUR
Availability: InStock
Rating: 3 (1 reviews)

Product no: AS14 2805A

AS14 2805A | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

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DATA SHEET IN PDF

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Data sheet
Product Info

Immunogen:	UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 500-1 : 5000 (WB)
Expected \| apparent MW:	75 \| 100 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Brassicaceae family, Solanum lycopersicum Species of your interest not listed? Contact us
Not reactive in:	Citrus sinensis, Nicotiana tabacum

Additional Information

Additional information (application):

A specific extraction method and tissue type needs to be used as described in Minina et al, (2013), Dilution in western blot depends upon amount of NBR1 in the sample

Background

Background:

NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin.

Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.

Protocols
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Number of reviews: (1)

Monika | 2019-11-24

0 | 0

Western blotting: even after ON blocking of PVDF membrane in leaf extracts from Arabidopsis some strong non-specific bands are visible. Dilution of the antibody 1:1000 worked fine, 1:5000 was too low.