Name:
Phone:
E-mail:
Address:






p15 [Peanut clump virus]

AS16 3983 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: p15 [Peanut clump virus]

p15 [Peanut clump virus] in the group Antibodies Plant/Algal  / Plant Pathogens / Viruses at Agrisera AB (Antibodies for research) (AS16 3983)
p15 [Peanut clump virus]



DATA SHEET IN PDF

Qty: 
292
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant p15 [Peanut clump virus] Protein accession number: NP_620041.
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW 14,6 | 15 kDa

Reactivity

Confirmed reactivity p15 [Peanut clump virus]
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-p15 [Peanut clump virus] antibodies

The indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions. Exposure time was 20 seconds.

Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA

Additional information

Related products

Background

Product citations

Related products: p15 [Peanut clump virus]

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volum...
From 15 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
199 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €