PDH-E1 BETA | Pyruvate dehydrogenase E1 component subunit beta-2 (chloroplastic)

AS22 4835   | Clonality: Polyclonal|  Host: Rabbit | Reactivity: Arabidopsis Thaliana, Capsicum annuum, Solanum tuberosum, Solanum lycopersicum, Brassica napus

PDH-E1 BETA | Pyruvate dehydrogenase E1 component subunit beta-2 (chloroplastic) in the group Antibodies Plant/Algal  / Capsicum annuum at Agrisera AB (Antibodies for research) (AS22 4835)

Data sheet Protocols Add review
background: Background info.
Picture (footer): application example

µg of total protein extracted freshly from    with write exact buffer components ..... and denatured °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

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calculated | apparent molecular mass [kDa]: 44.2 | 39.5 kDa kDa (due to N-terminal or C-terminal processing)






immunogen: KLH-conjugated peptide derived from Q9C6Z3
Purity: Affinity purified serum, in PBS pH 7.4
Quantity: 50 µg
recommended dilution: 1 : 1000 (WB)
Reconstitution: For reconstitution add 50 µl, of sterile or deionized water.
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications: Western blot (WB)
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity:
Capsicum annuum
Solanum tuberosum
Solanum lycopersicum
Brassica napus

Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known

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