PDH-E1 BETA | Pyruvate dehydrogenase E1 component subunit beta-2 (chloroplastic)

µg of total protein extracted freshly from    with write exact buffer components ..... and denatured with....at °C or 70°C for 2-5 min.... were separated on  % SDS-PAGE  and blotted 1h to PVDF/nitrocellulose using wet, semi-dry or dry transfer. Blot was blocked with % milk or % BSA  for 1h/RT or 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in  for 1h/RT with agitation. The blot was washed as above and developed for   min with   name and supplier. Exposure time was.....seconds.

Courtesy of

Polyclonal

Lyophilized

Rabbit

Capsicum annuum

Solanum tuberosum

Solanum lycopersicum

Brassica napus