PetL | Cytochrome b6-f complex subunit 6

Product no: AS22 4883

AS22 4883 | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Nicotiana tabacum

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Nicotiana tabacum PetL protein sequence, UniProt: P69401
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 3.4 | kDa (due to N-terminal or C-terminal processing)
  • Reactivity
  • Confirmed reactivity: Nicotiana tabacum
    Predicted reactivity: Arabidopsis thaliana, Elsholtzia minima, Plantago ovata, Solanum aloysiifolium, Solanum lycopersicum, Solanum tuberosum, Zea mays

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot detection using anti-PetL antibodies

    8-16 µg/well of thylakoids extracted from Nicotiana tabacum. Exact buffer components were: 50 mM Hepes-KOH pH 7.5, 100 mM sorbitol, 10 mM MgCl2 and denatured with 0.1 M Tris/HCl pH 6.8, 0.24% glycerol, 8% SDS, 2% b-mercaptoethanol, 0.2 mg/ml Coomassie Blue G250 at 95 °C 10 min.  Samples were separated in the cold on 10 % SDS-tricine and blotted for overnight to PVDF, using wet transfer in the cold. Blot was blocked with 4 % milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:  500 for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 10 min and 3 times in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1: 25 000 1h/RT for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AgriseraBright, AS16 ECL-N. Exposure time was 25 seconds.

  • Background
  • Background: PetL (Cytochrome b6-f complex subunit 6) is a part of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. The protein is of importance for photoautotrophic growth as well as for electron transfer efficiency and stability of the cytochrome b6-f complex. Alternative name: Cytochrome b6-f complex subunit VI
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
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AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. rapa subsp. chinensis, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, S. oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Z. mays

Benefits of using this antibody

319 €
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS22 4711   | Clonality:  Polyclonal  |  Host:  Rabbit  | Reactivity: Arabidopsis thaliana, Synechocystis sp. , Synechococcus sp., Cyanobacterium aponinum, Chlorogloeopsis sp.

319 €
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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AS16 ECL-N |  low pico to mid femtogram detection | Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-N-10, 10 ml. Trial size limited to one per customer

AS16 ECL-N-100, 100 ml

Choose the appropriate volume in the drop down menu to the right

From 13 €