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Phly | DNA photolyase (At4g25290) (N-terminal part)

AS15 2863   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

Phly | DNA photolyase (At4g25290) (N-terminal part) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Cell cycle  at Agrisera AB (Antibodies for research) (AS15 2863)

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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana DNA photolyase, UniProt: F4JSJ6, TAIR: AT4G25290, loacted in the N-terminal part of the protein
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

78 | 90 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-Phyl antibodies

2,5 µg of total protein from Arabidopsis thaliana wilde type darkness (1), wilde type light (2) and insertion mutants:  SALK_056328C darkness (3), SALK_056328C light (4), extracted with 0.1 M Tris-HCl pH 8.5, 4% SDS, 2% (v/v) 2-mercaptoethanol, 2 mM phenylmethylsulfonyl fluoride and denatured with Laemmli buffer at 95oC for 10 min were separated on 12% SDS-PAGE and blotted 2h to PVDF using semi-dry transfer. Blots were blocked with 5% milk PBS-T (Tween 0.5%) for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in 5% milk PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:25 000 in 5 %milk PBS-T for 1h at RT with agitation. The blot was rinsed briefly twice, then washed 3 times for 10 min in PBS-T at RT with agitation. Blot was developed for 5 min with chemiluminescent detection reagent. Exposure time was 5 minutes.

Courtesy of Dr. Justyna Łabuz, Department of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Poland

Additional information

Related products

Related products

AS15 2864 | Anti-Phly | DNA photolyase (At4g25290), rabbit antibodies (peptide chosen to elicit this antibody is located towards C-terminal part of the protein)

collection of antibodies to DNA/RNA cell cycle

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background Protein belongs to DNA photolyases and functions in DNA repair.

Product citations

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